Treatment of injuries in javelin horses with propolis
Abstract
Debridement and suturing of deep and infected wounds in horses requires sedation and anesthesia due to the limitations of horses. For this reason, it is preferred to perform wound treatment and care with more practical methods. The aim of this study is to evaluate wound healing in 18 horses by utilizing the antimicrobial, anti– inflammatory and local anesthetic effects of propolis. Various wounds resulting from trauma on 18 male, Arabian and British horses aged between 3-8 at Malatya Equestrian Javelin Club were treated by applying liquid propolis in spray form for 5 days. Wound size was measured with a ruler on days 1, 3, 5, 9, 13, 15 and 21 after the application. All wounds started to heal on the 3rd day after the application, graze wounds healed on the 9th day, and deep wounds healed on the 15th day. It was concluded that wound treatment with spray propolis in horses can be preferred because it is easy to apply, economical, creates biofilm on the wound and provides healing in a short time.
Downloads
References
Samsar E, Akın F. Genel Cerrahi (Veteriner) [Veterinary general surgery]. Malatya (Türkiye): Medipres; 2003. 442 p. Turkish.
Shetty V, Schwartz HC. Wound healing and perioperative care. Oral maxillofac. Surg. Clin. North Am. [Internet]. 2006; 18(1):107-113. doi: https://doi.org/d98rj5 DOI: https://doi.org/10.1016/j.coms.2005.09.004
Keskin M, Kolaylı S. Standardization of propolis, Is it possible?. U. Bee J. [Internet]. 2018; 18(2):101-110. doi: https://doi.org/pm8z
Castaldo S, Capasso F. Propolis, an old remedy used in modern medicine. Fitoterapia. [Internet]. 2002; 73(1):S1-S6. doi: https://doi.org/fxhbrb DOI: https://doi.org/10.1016/S0367-326X(02)00185-5
Acaroz U, Kurek–Gorecka A, Olczyk P, Tas N, Ali A, Paramanya A, Balyan, P, Noor A, Kamaraj S, Malekıfard F, Hosseını A, Istanbullugıl FR, Arslan–Acaroz D, Asma ST, Seguenı N, Ceylan AB, Jin X. The role of bee products in the control of antimicrobial resistance and biofilm formation Kafkas Univ. Vet. Fak. Derg. [Internet]. 2024. 30(2):131-153. doi: https://doi.org/pm83 DOI: https://doi.org/10.9775/kvfd.2023.30966
Doğan N, Hayoğlu İ. Propolis ve kullanım alanları [Propolis and areas of usage]. Harran Tarım Ve Gıda Bilim. Derg. [Internet]. 2012 [cited 18 Dec. 2024]; 16(3):39-48. Turkish. Available in: https://goo.su/mf3NvLv
Hendrickson DA. Management of superficial wounds. In: Auer JA, Stick JA, editors. Equine Surgery. 3rd Ed. Missouri (USA): Elsevier Saunders; 2006. p. 288-298. DOI: https://doi.org/10.1016/B1-41-600123-9/50028-0
Stadelmann WK, Digenis AG, Tobin GR. Physiology and healing dynamics of chronic cutaneous wounds. Am. J. Surg. 1998; 176(2):26-38. doi: https://doi.org/fdjbt9 DOI: https://doi.org/10.1016/S0002-9610(98)00183-4
Howaida AA, Abdel–Wahed RE, Al–Kammar MH. El–Neweshy MS. Evaluation of the effectiveness of propolis compared with honey on second intention wound healing in the equine. Middle East J. Sci. Res. [Internet]. 2013 [cited 23 Dec. 2024]; 14(10):1292-1298. Available in: https://n9.cl/d76uhs
Stashak TS, Theoret C. Equine wound management. 2nd Ed. Oxford (UK): Blackwell Publishing; 2008. 694 p.
Carnwath R, Graham EM, Reynolds K, Pollock PJ. The antimicrobial activity of honey against common equine wound bacterial isolates. Vet. J. [Internet]. 2014; 199(1):110-114. doı: https://doi.org/f5s679 DOI: https://doi.org/10.1016/j.tvjl.2013.07.003
Alford CG, Caldwell FJ, Hanson R. Equine distal limb wounds: New and emerging treatments. Compend. Contin. Educ. Vet. [Internet]. 2012 [cited 23 Dec. 2024]; 34(7):E5. PMID: 22847323. Available in: https://goo.su/I4hW1v
Ulma RM, Aghaloo TL, Freymiller EG. Chapter 2 – Wound Healing. In: Fonseca RJ, Walker RV, Barber, HD, Powers MP, Frost DE, editors. Oral and Maxillofacial Trauma. Philadelphia (USA): Saunders; 2013. p. 9–29. DOI: https://doi.org/10.1016/B978-1-4557-0554-2.00002-2
Theoret CL, Wilmink JM. Aberrant wound healing in the horse: naturally occurring conditions reminiscent of those observed in man. Wound Repair Regen. [Internet]. 2013; 21(3):365–371. doi: https://doi.org/f4ww9m DOI: https://doi.org/10.1111/wrr.12018
Akgül MB, Başer E, Gülaydın A, Şındak NA. Bir Atta Blanket’e Bağlı Kuyruk Yaralanması Olgusu [The case of tail wound depend on blanket injury in a horse]. Uludag Univ. J. Fac. Vet. Med. 2016; 35(1-2):49-52. Turkish. doi: https://doi.org/png9 DOI: https://doi.org/10.30782/uluvfd.393928
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am. J. Enol. Vitic. [Internet]. 1965; 16:144–158. doi: https://doi.org/gsth7k DOI: https://doi.org/10.5344/ajev.1965.16.3.144
Singleton VL, Orthofer R, Lamuela–Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin–ciocalteu reagent. Meth. Enzymol. [Internet]. 1999; 299:152–178. doi: https://doi.org/b28jxj DOI: https://doi.org/10.1016/S0076-6879(99)99017-1
Fukumoto LR, Mazza G. Assessing antioxidant and prooxidant activities of phenolic compounds. J. Agricul. Food Chem. [Internet]. 2000; 48(8):3597–3604. doi: https://doi.org/b2fdsf DOI: https://doi.org/10.1021/jf000220w
Keast DH, Bowering CK, Evans AW, Mackean GL, Burrows C, D’Souza L. Measure: A proposed assessment framework for developing best practice recommendations for wound assessment. Wound Repair Regen. [Internet]. 2004; 12(1):S1–S17. doi: https://doi.org/dz2r76 DOI: https://doi.org/10.1111/j.1067-1927.2004.0123S1.x
Caston SS. Wound care in horses. Vet. Clin. North Am. Equine Pract. [Internet]. 2012; 28(1):83–100. doi: https://doi.org/f336pm DOI: https://doi.org/10.1016/j.cveq.2012.01.001
van Rijswijk L, Braden BJ. Pressure ulcer patient and wound assessment: an AHCPR clinical practice guideline update. Ostomy Wound Manag. 1999; 45(1A):56S–69S. PMID: 10085976.
Kolaylı S, Kara Y, Can Z. Comparative study of some commercial propolis extract with new prepared ethanolic propolis extract. Bee Studies [Internet]. 2020; 12(2):27–30. doi: https://doi.org/pnhb DOI: https://doi.org/10.51458/BSTD.2021.5
Oruç HH, Sorucu A, Ünal HH. Aydın L. Effect of season and altitude on the levels of certain biologically active phenolic compounds in propolis and partial standardization of propolis. Ankara Üniv. Vet. Fak. Derg. [Internet]. 2017; 64(1):13–20. doı: https://doi.org/pnhc DOI: https://doi.org/10.1501/Vetfak_0000002767
Bakchiche B, Habati M, Benmebarek A, Gherib A. Total phenolic, flavonoid contents and antioxidant activities of honey and propolis collected from the region of Laghouat (South of Algeria). World News Nat. Sci. [Internet]. 2017; 11:91–97. doi: https://doi.org/pnhd DOI: https://doi.org/10.23880/IPCM-16000110
Can Z, Yildiz O, Sahin H, Turumtay EA, Silici S, Kolayli S. An investigation of Turkish honeys:their physico–chemical properties, antioxidant capacities and phenolic profiles. Food. Chem. [Internet]. 2015; 180:133–141. doi: https://doi.org/f7wqf4 DOI: https://doi.org/10.1016/j.foodchem.2015.02.024
Temizer İK, Güder A, Çelemli ÖG. Botanical origin and antioxidant activities of propolis from the Irano–Turanian region. Istanbul J. Pharm. [Internet]. 2017; 47(3):107–111. doi: https://doi.org/pnhf
Simoni IC, Aguiar B, Navarro AMA, Parreira RM, Fernandes MJB, Sawaya ACHF, Fávero OA. In vitro antiviral activity of propolis and Baccharis sp. extracts on animal herpesviruses. Arq. Inst. Biol. [Internet]. 2018; 85(1–7):e0972016. doi: https://doi.org/pnhg DOI: https://doi.org/10.1590/1808-1657000972016
Eggleston RB. Equine wound management: Bandages, casts, and external support. Vet. Clin. North Am. Equine Pract. [Internet]. 2018; 34(3):557–574. doi: https://doi.org/gfs9x6 DOI: https://doi.org/10.1016/j.cveq.2018.07.010
Nada AA, Nour IH, Metwally AM, Asaad AM, Shams Eldin SM, Ibrahim RS. An integrated strategy for chemical, biological and palynological standardization of bee propolis. Microchemical J. [Internet]. 2022; 182(2):107923. doi: https://doi.org/pnhh DOI: https://doi.org/10.1016/j.microc.2022.107923
Rodríguez ISF, Monteagudo MM, Orozco AL, Sánchez TAC. Use of mexican propolis for the topical treatment of dermatomycosis in horses. Open J. Vet. Med. [Internet]. 2016; 6(1):1–8. doi: https://doi.org/pnhj DOI: https://doi.org/10.4236/ojvm.2016.61001