Efectos de la Clitoria Ternatea en la lesión renal por isquemia y reperfusión en ratas

• Şule Melek Bingol Universıty, Faculty of Veterinary Medicine, Department of Surgery. Bingöl, Turkey. https://orcid.org/0000-0002-0677-722X
• Ahmet Uyar Hatay Mustafa Kemal University, Faculty of Veterinary Medicine Department of Pathology. Hatay, Turkey. https://orcid.org/0000-0001-9694-8629
• Hürrem Turan Akkoyun Siirt University, Faculty of Veterinary Medicine, Department of Physiology. Siirt, Turkiye. https://orcid.org/0000-0002-4547-8003
• Serdar Altun Ataturk University, Faculty of Veterinary Medicine, Department of Pathology. Erzurum, Turkiye. https://orcid.org/0000-0002-8735-7031
• Mahire Bayramoğlu Akkoyun Siirt University, Faculty of Veterinary Medicine, Department of Biochemistry, Siirt, Turkiye. https://orcid.org/0000-0001-5150-5402
• Fatma Karagözoğlu Dokuz Eylul University, Faculty of Veterinary Medicine, Department of Anımal Nutrıtıon and Nutritional Diseases. İzmir, Turkiye. https://orcid.org/0000-0001-7970-0306
• Suat Ekin Van Yüzüncü Yıl University, Faculty of Science, Department of Chemistry. Van, Turkiye. https://orcid.org/0000-0002-6502-5028
• Aydın Şükrü Bengü Bingöl University, Vocational School of Health Services. Bingöl, Turkiye. https://orcid.org/0000-0002-7635-4855
• Gültekin Atalan Erciyes Universıty, Faculty of Veterinary Medicine, Department of Surgery. Kayseri, Turkiye. https://orcid.org/0000-0002-2613-5638
• Ahmet Bakir YYU University, Faculty of Sciences, Department of Chemistry. Van, Turkiye. https://orcid.org/0000-0003-0797-285X

DOI: https://doi.org/10.52973/rcfcv-e35657

Palabras clave: Riñón, isquemia-reperfusión, rata, Clitoria ternatea, terapeutico

Resumen

La lesión por isquemia-reperfusión (I/R) se refiere al daño causado por la interrupción temporal del flujo sanguíneo en un órgano/tejido y el restablecimiento de la circulación. Esta lesión, que afecta a muchos  órganos  vitales,  sigue  siendo un importante problema clínico y quirúrgico. Este estudio pretende  investigar el efecto antioxidante y antiinflamatorio de la Clitoria ternatea sobre la lesión renal por I/R en ratas. Se agruparon 24 ratas albinas Wistar macho en 4 grupos, 6 ratas en cada uno (Control, grupo I/R, grupo Clitoria ternatea, grupo I/R + grupo Clitoria ternatea). Al final de los procedimientos experimentales, se practicó la eutanasia a todos los grupos de acuerdo con las normas éticas. Se tomaron muestras de sangre y tejidos y los posteriores analisis bioquímicas (MDA, SOD, CAT, GSH-Px, BUN, Na, K, creatina), histopatológicas e inmunohistoquímicas demostraron que la Clitoria ternatea prevenia eficazmente la lesión renal inducida por I/R en ratas. Basandose en estos resultados, la Clitoria ternatea puede considerarse un agenta terapeutico potenscial para la prevencion del dano renal inducido por I/R. 

Citas

Gültekin Ç, Sayiner S, Çetinel Ş, Şehirli AÖ. Does Ambroxol alleviate kidney ischemia-reperfusion injury in rats?. Iran. J. Basic Med. Sci. [Internet]. 2022; 25(8):1037-1041. doi: https://doi.org/psbn

Deng Y, Li R, Yang YL, Weiss S, Smith P. Pharmacological prevention of renal ischemia–reperfusion injury in a rat model. ANZ J. Surg. [Internet]. 2022; 92(3):518-525. doi: https://doi.org/psbq DOI: https://doi.org/10.1111/ans.17381

Yuzbasioglu MF, Aykas A, Kurutas EB, Sahinkanat T. Protective effects of propofol against ischemia/reperfusion injury in rat kidneys. Ren. Fail. [Internet]. 2010; 32(5):578-583. doi: https://doi.org/cx23h6 DOI: https://doi.org/10.3109/08860220903548940

Uyar A, Yaman T. Evaluation with endothelial nitric oxide synthase (eNOS) immunoreactivity of the protective role of astaxanthin on hepatorenal injury of remote organs caused by ischaemia reperfusion of the lower extremities. Prz. Gastroenterol. [Internet]. 2020; 15(2):161-172. doi: https://doi.org/psbr DOI: https://doi.org/10.5114/pg.2019.88620

Oguis GK, Gilding EK, Jackson MA, Craik DJ. Butterfly pea (Clitoria ternatea), a cyclotide-bearing plant with applications in agriculture and medicine. Front. Plant. Sci. [Internet]. 2019; 10:645. doi: https://doi.org/gnkqd5 DOI: https://doi.org/10.3389/fpls.2019.00645

Safhi FA, ALshamrani SM, Jalal AS, Awad NS, Sabit H, Abdelgawad FE, Khalil SS, Khodeer DM, Mobasher MA. Asian Pigeonwing Plants (Clitoria ternatea) Synergized Mesenchymal Stem Cells by Modulating the Inflammatory Response in Rats with Cisplatin-Induced Acute Kidney Injury. Pharmaceuticals. [Internet]. 2022; 15(11):1396. doi: https://doi.org/psb6 DOI: https://doi.org/10.3390/ph15111396

Vidana-Gamage GC, Lim YY, Choo WS. Anthocyanins from Clitoria ternatea flower: Biosynthesis, extraction, stability, antioxidant activity, and applications. Front. Plant Sci. [Internet]. 2021; 12:792303. doi: https://doi.org/psb7 DOI: https://doi.org/10.3389/fpls.2021.792303

Saraç M. Deneysel Spinal Kord Hasarında Clitoria Ternatea (Mavi Kelebek Sarmaşığı) Ekstraktının Nöroprotektif Ve Terapötik Etkisinin Araştırılması. [master’s thesis on the Internet] T.C.Bingöl Üniversitesi Sağlık Bilimleri Enstitüsü Veterinerlik Cerrahisi Anabilim Dalı. Bingöl, 2024; 62 p. Available in: https://goo.su/EgiQD

Abbas WJ, Altemimi ML, Al-Mudhafar Zigam QA, Hadi NR. Effects of Vinpocetine on Renal Ischemia Reperfusion Injury in a Male Rat Model. Sys. Rev. Pharm. [Internet]. 2020[cited 15 Feb 2025]; 11(12):2380-2389. Available in: https://goo.su/zxcXqx2

Nithianantham K, Yuet-Ping K, Yoga-Latha L, Jothy SL, Darah I, Chen Y, Chew AL, Sasidharan S. Evaluation of hepatoprotective effect of methanolic extract of Clitoria ternatea (Linn.) flower against acetaminophen-induced liver damage. Asian. Pac. J. Trop. Dis. [Internet]. 2013; 3(4):314-319. doi: https://doi.org/psb8 DOI: https://doi.org/10.1016/S2222-1808(13)60075-4

Xia E, Rao G, Van-Remmen H, Heydari AR, Richardson A. Activities of antioxidant enzymes in various tissues of male Fischer 344 rats are altered by food restriction. J Nutr. [Internet]. 1995; 125(2):195-201. doi: https://doi.org/pp6g DOI: https://doi.org/10.1093/jn/125.2.195

Yaman T, Akkoyun HT, Bayramoglu-Akkoyun M, Karagözoglu F, Melek S, Keles ÖF, Bengü AS. Assessment of the effect of sodium tetraborate on oxidative stress, inflammation, and apoptosis in lead-induced nephrotoxicity. Drug Chem. Toxicol .[Internet]. 2025; 48(1):150-162. doi: https://doi.org/psb9 DOI: https://doi.org/10.1080/01480545.2024.2358067

Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin. Chem. [Internet]. 1988; 34(3):497–500. doi: https://doi.org/gj74fn DOI: https://doi.org/10.1093/clinchem/34.3.497

Gallo G, Martino G. The Spectrophotometric method to Measure Glutathione Peroxidase activity in Human and Rat Red Blood Cells. In: Arti Prasad, ed. Current Res. Life Sci. AkiNik Publications. 2023; p. 25-34. doi: https://doi.org/pscb

Omidpanah N, Ebrahimi S, Raygani AV, Mozafari H, Rezaei M. Total antioxidant capacity, catalase activity and salivary oxidative parameters in patients with temporo mandibular disorders. Front Dent. [Internet]. 2020; 17(16):1-6. doi: https://doi.org/pscc DOI: https://doi.org/10.18502/fid.v17i16.4179

Ohkawa H, Ohishi N, Yagi K. Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction. Anal. Biochem. [Internet]. 1979; 95(2):351-358. doi: https://doi.org/bktx4x DOI: https://doi.org/10.1016/0003-2697(79)90738-3

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951 [cited Apr 15 2025]; 193(1):265–275. Available in: https://goo.su/PDPidR DOI: https://doi.org/10.1016/S0021-9258(19)52451-6

Luna-Lee G. Manual of histologic staining methods of the Armed Forces Institute of Pathology. 3rd ed. USA: McGraw-Hill Book Company; 1968 [cited Apr 15 2025]. Available in: https://goo.su/l29gwMr

Ozan E, Koyutürk L, Sapmaz T. Böbrek Iskemi-Reperfüzyon Hasarinda Antioksidan Olarak Prostoglandin E1 (PGE1) Kullaniminin Incelenmesi: Deneysel Çalisma. Firat Tip Dergisi. [Internet]. 2004 [cited Jan 22 2025]; 9(3):67-71. Available in: https://goo.su/eazRyU

Baratta JL, Ngo A, Lopez B, Kasabwalla N, Longmuir KJ, Robertson RT. Cellular organization of normal mouse liver: a histological, quantitative immunocytochemical, and fine structural analysis. Histochem. Cell. Biol. [Internet]. 2009; 131:713–726. doi: https://doi.org/b72mpt DOI: https://doi.org/10.1007/s00418-009-0577-1

Mousavi G. Study on the effect of black cumin (Nigella sativa Linn) on experimental renal ischemia-reperfusion injury in rats. Acta Cir. Bras. [Internet]. 2015; 30(8):542-550. doi: https://doi.org/gq2m3x DOI: https://doi.org/10.1590/S0102-865020150080000005

Wu S, Guo X, Shang J, Li Y, Dong W, Peng Q, Xie Z, Chen C. Effects of Lycopene Attenuating Injuries in Ischemia and Reperfusion. Oxid. Med. Cell. Longev. [Internet]. 2022; 2022:9309327. doi: https://doi.org/pssm DOI: https://doi.org/10.1155/2022/9309327

Ortadeveci A, Oz S. A Review on Renal Ischemia and Reperfusion Injury. Osm. Med. J. [Internet]. 2017; 39(3):115-124. doi: https://doi.org/pssn

Unal D, Yeni E, Erel O, Bitiren M, Vural H. Antioxidative effects of exogenous nitric oxide versus antioxidant vitamins on renal ischemia reperfusion injury. Urol. Res. [Internet]. 2002; 30:190–194. doi: https://doi.org/bp45n4 DOI: https://doi.org/10.1007/s00240-002-0254-5

Sahna E, Parlakpinar H, Ozturk F, Cigremis Y, Acet A. The protective effects of physiological and pharmacological concentrations of melatonin on renal ischemiareperfusion injury in rats. Urol. Res. [Internet]. 2003; 31:188–193. doi: https://doi.org/cxf53t DOI: https://doi.org/10.1007/s00240-003-0314-5

Demirtas O, Kaya M, Boleken ME, Kanmaz T,Çelik H, Baba F, Erel Ö, Yücesan S. Böbrek iskemi reperfüzyon hasarinda pentoksifilinin etkisi. Turk. J. Pediatr. Surg. [Internet]. 2010 [cited Feb 15 2025]; 24(1):1-6. Available in: https://goo.su/ptEeK

Sentürk H, Kolankaya D, Sahin Y. Renal Iskemi-Reperfüzyonu Sirasinda Siçan Böbreginde Olusan Oksidatif Stres Hasarina Silimarin Etkisi.Çankaya Univ. J. Sci. Eng. 2010 [cited Feb 15 2025]; 7(1). Available in: https://goo.su/6CdIV

Jeyaraj EJ, Lim YY, Choo WS. Antioxidant, cytotoxic, and antibacterial activities of Clitoria ternatea flower extracts and anthocyanin-rich fraction. Sci. Rep. [Internet]. 2022; 12:14890. doi: https://doi.org/kjmh DOI: https://doi.org/10.1038/s41598-022-19146-z

Islam MA, Mondal SK, Islam S, Akther-Shorna MN, Biswas S, Uddin MS, Zaman S, Saleh MA. Antioxidant, cytotoxicity, antimicrobial activity, and in silico analysis of the methanolic leaf and flower extracts of Clitoria ternatea. Biochem. Res. Int. [Internet]. 2023; 2023(1):8847876. doi: https://doi.org/pssq DOI: https://doi.org/10.1155/2023/8847876

Grekas D, Dioudis C, Papageorgiou G, Iliadis S, Zilidis C, Alivanis P, Dimitriadou A, Tourkantonis A. Lipidperoxidation after acute renal ischemia and reperfusion in rats: The effect of trimetazidine. Ren. Fail. [Internet]. 1996; 18(4):545-552. doi: https://doi.org/dc3j6w DOI: https://doi.org/10.3109/08860229609047676

Shiravi A, Jalili C, Vaezi G, Ghanbari A, Alvani A. Acacetin attenuates renal damage-induced by ischemiareperfusion with declining apoptosis and oxidative stress in mice. Int. J. Prev Med. [Internet]. 2020; 11(1):22. doi: https://doi.org/pssr DOI: https://doi.org/10.4103/ijpvm.IJPVM_512_18

Ascon DB, Lopez-Briones S, Liu M, Ascon M, Savransky V, Colvin RB, Soloski MJ, Rabb H. Phenotypic and functional characterization of kidney-infiltrating lymphocytes in renal ischemia reperfusion injury. J. Immunol. [Internet]. 2006; 177(5):3380–3387. doi: https://doi.org/gn3p37 DOI: https://doi.org/10.4049/jimmunol.177.5.3380

Hou S, Yan X, Gao X, Jockusch S, Gibson KM, Shan Z, BiL. Enhancing Cardiomyocyte Resilience to Ischemia-Reperfusion Injury: The Therapeutic Potential of anIndole-Peptide-Tempo Conjugate (IPTC). ACS Omega. [Internet]. 2024; 9(38):39401-39418. doi: https://doi.org/pssw DOI: https://doi.org/10.1021/acsomega.4c02725

Aydoğdu N, Kaymak K, Yalçın Ö. Effects of n-acetylcysteine in renal ischemia/reperfusion injury in the rats. Firat Med. J. [Internet]. 2005 [cited Jan 12 2025]; 10(4):151-155. Available in: https://goo.su/EgDg

Cuan J, Wu C, Zheng F, Lu X, Umair waqas M. Sevoflurane Pretreatment Enhances Myocardial Ischemia-Reperfusion Injury Via Activating HIF-1a/iNOS/cGMP to Inhibit Endoplasmic Reticulum Stress. Pak. Vet. J. [Internet]. 2024; 44(3):785-793. doi: https://doi.org/pssx

Sarumathy K, Rajan MD, Vijay T, Jayakanthi J. Evaluation of phytoconstituents, nephro-protective and antioxidant activities of Clitoria ternatea. J. Appl. Pharm. Sci. [Internet]. 2011 [cited March 01 2025]; 01(05)164-172. Available in: https://goo.su/hBITnUB

De Groot H, Rauen U. Ischemia-reperfusion injury: processes in pathogenetic networks: A review. Transplant. Proc. [Internet]. 2007; 39(2):481-484. doi: https://doi.org/dzwrpz DOI: https://doi.org/10.1016/j.transproceed.2006.12.012

Oh MS, Briefel G, Pincus MR. Evaluation of renal function, water, electrolytes, and acid-base balance. In: McPherson RA, Pincus MR, eds. Henry’s Clinical Diagnosis and Management by Laboratory Methods. 24th ed. Philadelphia, PA: Elsevier; 2022. p. 182-207.

Ahmadi F, Hajihashemi S, Rahbari A, Ghanbari F. Therapeutic effects of hydralazine on renal ischemia- reperfusion injury in rat. J. Contemp. Med. Sci. [Internet]. 2019 [cited Feb 11 2025]; 5(6):317-324. Available in: https://goo.su/wfbWYv DOI: https://doi.org/10.22317/jcms.v5i6.622

Sagiroglu T, Torun N, Yagci M, Yalta T, Sagiroglu G, Oguz S. Effects of apelin and leptin on renal functions following renal ischemia/reperfusion: An experimental study. Exp. Ther. Med. [Internet]. 2012; 3(5):908-914. doi: https://doi.org/pssz DOI: https://doi.org/10.3892/etm.2012.499

Hacıoğlu C, Kar F, Şentürk H, Kanbak G. Effects of boric acid on electrolyte balance and lipid profile against renal ischemia reperfusion injury. Biol. Divers. Conserv. [Internet]. 2018 [cited Dec 17 2024]; 11(2):76-81. Available in: https://goo.su/oi10

Priante G, Gianesello L, Ceol M, Prete DD, Anglani F. Cell death in the kidney. Int. J. Mol. Sci. [Internet]. 2019; 20(14):3598 doi: https://doi.org/ghcm5m DOI: https://doi.org/10.3390/ijms20143598

Öğütmen MB. Akut böbrek yetmezliği. GKD Anest. Yoğ. Bak. Dern. Derg. [Internet]. 2011; 17(2):25-33. doi: https://doi.org/pss2

Lameire N, Van Biesen W, Vanholder R. Acute renal failure. Lancet [Internet]. 2005; 365(9457):417-430. doi: https://doi.org/b52f5w DOI: https://doi.org/10.1016/S0140-6736(05)17831-3

Akkoç A. Ratlarda böbrek iskemi-reperfüzyon hasarında proflaktik kalsiyum dobesilatın etkinliği. [medical specialization thesis on the Internet]. Türkçe: Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Üroloji Anabilim Dalı, Bolu. 2010 [cited Feb 15 2025]; 84 p. Available in: https://goo.su/zNVCEN

Thiemermann C, Patel NSA, Kvale EO, Cockerill GW, Brown PAJ, Stewart KN, Cuzzocrea S, Britti D, Mota-Filipe H, Chatterjee PK. High Density Lipoprotein (HDL) Reduces Renal Ischemia/Reperfusion Injury. J. Am. Soc. Nephrol. [Internet]. 2003; 14(7):1833-1843. doi: https://doi.org/fcg6br DOI: https://doi.org/10.1097/01.ASN.0000075552.97794.8C

Brady HR, Singer GG. Acute renal failure. Lancet [Internet].1995; 346(8989):1533–1540. doi: https://doi.org/c3zpjr DOI: https://doi.org/10.1016/S0140-6736(95)92057-9

Conesa LE, Valero F, Nadal JC, Fenoy FJ, López B, Arregui B, Salom MG. N-Acetyl-L-Cysteine improves renal medullary hypoperfusion in acute renal failure. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2001; 281(3):R730-R737. doi: https://doi.org/pss4 DOI: https://doi.org/10.1152/ajpregu.2001.281.3.R730

Malek M, Nematbakhsh M. Renal ischemia/reperfusion injury; from pathophysiology to treatment. J. Renal Inj. Prev. [Internet]. 2015; 4(2):20-27. doi: https://doi.org/ggsdtf

Ozturk H, Ozturk H, Terzi EH, Özgen U, Duran A, UygunI. Protective effects of Rosmarinic acid against renal ischemia/reperfusion injury in rats. J. Pak. Med. Assoc. [Internet]. 2014 [cited March 5 2025]; 64(3):260-265. Available in: https://goo.su/BzU28oE

Rodríguez-Reynoso S, Leal C, Portilla-de Buen E, Castillo JC, Ramos-Solano F. Melatonin ameliorates renal ischemia/reperfusion injury. J. Surg. Res. [Internet]. 2004; 116(2):242-247. doi: https://doi.org/cchp2p DOI: https://doi.org/10.1016/j.jss.2003.10.002

Sener G, Sehirli AO, Keyer-Uysal M, Arbak S, Ersoy Y, Yeğen BÇ. The protective effect of melatonin on renal ischemia-reperfusion injury in the rat. J. Pineal Res. [Internet]. 2002; 32(2):120-126. doi: https://doi.org/cj6gbr DOI: https://doi.org/10.1034/j.1600-079x.2002.1848.x

Aktoz T, Aydogdu N, Alagol B, Yalcin O, Huseyinova G, Atakan IH. The Protective Effects of Melatonin and Vitamin E against Renal Ischemia-Reperfusion Injury in Rats. Ren. Fail. [Internet]. 2007; 29(5):535-542. doi: https://doi.org/bx6mqd DOI: https://doi.org/10.1080/08860220701391738