Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race

• Ide Unchupaico-Payano Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa, Vicepresidencia de Investigación. La Merced, Perú
• Alberto Alponte-Sierra Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa, Vicepresidencia de Investigación. La Merced, Perú
• Carlos Quispe-Eulogio Universidad Peruana Los Andes, Escuela Profesional de Veterinaria y Zootecnia. Chorrillos, Huancayo, Perú
• Edith Ancco-Goméz Universidad Peruana Los Andes, Escuela Profesional de Veterinaria y Zootecnia. Chorrillos, Huancayo, Perú
• Alex Huamán-De La Cruz Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa, Vicepresidencia de Investigación. La Merced, Perú - Universidad Peruana Unión, Escuela Profesional de Ingeniería Ambiental. Lurigancho, Lima. Perú https://orcid.org/0000-0003-4583-9136
• Julio Mariño-Alfaro Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa, Vicepresidencia de Investigación. La Merced, Perú
• Alberto Patiño-Rivera Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa, Vicepresidencia de Investigación. La Merced, Perú
• Carmencita Lavado-Meza Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa, Vicepresidencia de Investigación. La Merced, Perú
• Lupe Huanca-Rojas Universidad Nacional Intercultural de la Selva Central Juan Santos Atahualpa, Vicepresidencia de Investigación. La Merced, Perú
• Luis Bazán-Alonso Universidad Nacional del Centro del Perú, Facultad de Ingeniería de Sistemas. El Tambo, Huancayo, Perú

DOI: https://doi.org/10.52973/rcfcv-e32157

Keywords: Sperm chromatin, membrane integrity, Peruvian rams

Abstract

The objective of this research was to evaluate the effect of sperm on chromatin stability and its relationship with the membrane integrity structural – physiological and the rate of fertilization of female sheep. Ejaculates of sperm (2 × 10⁹ sperm·mL^-1) with 70% of motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram was served with fifteen female sheep (n=75), generating thus five different Groups (A, B, C, D, and E). A control Group also was considered. Sperm nuclear chromatin stability (NCS) was evaluated using the Borate Buffer (BB), Sodium Dodecyl Sulfate (SDS), and the mixture of Ethylenediaminetetraacetic acid (EDTA) + SDS. The fertilization rate was evaluated after 16-18 hours post sperm injection. Sperm concentration showed a significant difference (P<0.05) between Groups. In Contrast, seminal volume, and sperm motility do not show a significant difference (P>0.05). A high correlation (r²=0.52) was observed between morphology and motility, and the fertilization rate was 74.6% (n=56). It was concluded in general that techniques to evaluate nuclear condensation values do have a high likelihood to give a diagnosis about the future potential of sperm populations in Junín ram.

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