Antioxidant and anti–inflammatory activities of Gallic acid in Japanese quails induced by oxidative stress

• Mehmet Mustafa İşgör Hatay Mustafa Kemal University, Faculty of Veterinary, Department of Biochemistry. Antakya, Turkey
• Altuğ Küçükgül Hatay Mustafa Kemal University, Faculty of Veterinary, Department of Biochemistry. Antakya, Turkey
• Sema Alaşahan Hatay Mustafa Kemal University, Faculty of Veterinary, Zootechnical department. Antakya, Turkey

DOI: https://doi.org/10.52973/rcfcv-e33258

Keywords: Gallic acid, growth performance, oxidative stress, inflammation, quails

Abstract

Gallic acid is a phenolic compound found in many plant sources with strong antioxidant activity. In this study, the bioactivity of Gallic acid was investigated in Japanese quails induced by oxidative stress. The study was performed on four groups of 40–day–old male Japanese quail (Coturnix japonica). Oxidative stress was created for 1 week by adding 0.5% hydrogen peroxide. The study was terminated by administering 100 mg·kg^-1 body weight Gallic acid intraperitoneally. Total antioxidant and total oxidant level analyzes from liver tissue homogenates were performed using a ready–made commercial kit. TNF–α levels from blood samples taken for anti–inflammatory activity were investigated by ELISA method. There were no statistically significant results on live weight gain between the experimental groups and control group. However, Gallic acid in liver homogenates together with H₂O₂ increased total antioxidant state (TAS) compared to H₂O₂ application, while it decreased total oxidant state (TOS) in the same groups. Moreover, while the oxidative stress index increased in the H₂O₂ group, it decreased significantly in both the Gallic acid and Gallic acid + H₂O₂ groups. Gallic acid application also caused regression in blood TNF–α expression levels, which were increased by H₂O₂ . In quails, Gallic acid showed antioxidant activity by increasing TAS levels and decreasing TOS levels, providing a significant decrease in oxidative stress index. It also provided anti–inflammatory activity by suppressing TNF–a levels. However, advanced molecular analyzes are needed to obtain more detailed information on the subject.

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