Growth, survival rate, body morphology and color of zebrafish (Danio rerio) exposed to Nicotine

• Koray Umut Yaraş İskenderun Technical University, Faculty of Marine Science and Technology. İskenderun, Hatay, Turkey
• Şehriban Çek–Yalniz İskenderun Technical University, Faculty of Marine Science and Technology. İskenderun, Hatay, Turkey

DOI: https://doi.org/10.52973/rcfcv-e33284

Keywords: Spinal curvature, Nicotine therapy, model organism

Abstract

Although Nicotine is one of the most potent toxins in the world, it has been used as replacement therapy and has been given to patients through gums, dermal patches, lozenges and inhalers. Zebrafish are excellent model organisms and are widely used in biomedical studies. The aim of this study was to evaluate the effects of Nicotine on the growth performance and survival rate of zebrafish larvae and juveniles. Four hundred and eighty (480) newly hatched larvae were divided into four experimental groups namely: Control (C, 0 mg·L^–1), N1 (N1, 5 mg·L^–1), N2 (N2, 10 mg·L^–1) and N3 (N3, 20 mg·L^–1) per liter of water. The results showed that Nicotine had an adverse effect on the growth and survival rate of zebrafish. In addition, morphological abnormalities were detected. The experimental groups exposed to Nicotine showed lower final weight and length compared to the control and were statistically significant. Of the dosage used in the present study, 20 mg·L^–1 Nicotine had the most negative effect on growth and survival rate. The survival rate decreased in all exposed groups compared to the control. The maximum growth and the highest survival rate were recorded in the control group. It is proposed that Nicotine when incorporated in water can adversely affect the body morphology, color, growth and survival rate of zebrafish. Cautions should be taken when used as replacement therapy.

References

Tyagi A, Sharma S, Wu K, Wu S, Xing–Liu, F, Zhao D, Deshpande RP, D’Agostino RB, Watabe K. Nicotine promotes breast cancer metastasis by stimulating N2 neutrophils and generating pre–metastatic niche in lung. Nat. Commun. [Internet]. 2021; 12:474. doi: https://doi.org/gjf85b

Lian S, Li S, Zhu J, Xia Y, Do Jung Y. Nicotine stimulates IL–8 expression via ROS/NF–κB and ROS/MAPK/AP–1 axis in human gastric cancer cells. Toxicol. [Internet]. 2022; 466:153062. doi: https://doi.org/kmnc

Pucci S, Fasoli F, Moretti M, Benfante R, Lascio SD, Viani P, Daga A, Gordon TJ, McIntosh M, Zoli M, Glementi F, Gotti C. Choline and Nicotine increase glioblastoma cell proliferation by binding and activating α7– and α9– containing nicotinic receptors. Pharmacol. Res. [Internet]. 2021; 163:105336. doi: https://doi.org/gn9wxk

Zoli M, Pucci S, Vilella A, Gotti C. Neuronal and extraneuronal nicotinic acetylcholine receptors. Curr. Neuropharmacol. [Internet]. 2018; 16:338–349. doi: https://doi.org/gdhfrz

Liu W, Tao ZW, Wang L, Yuan MLK, Liu L, Zhou S, Wei Y, Deng J, Liu HG, Yang M, Hu Y. Analysis of factors associated with disease outcomes in hospitalized patients with 2019 novel corona virus dieases. Chin. Med. J. [Internet]. 2020; 133:1032–1038. doi: https://doi.org/ggpxpn

Xavier J, Singh S, Kumari P, Ravichandiran V. Neurological repercussions of neonatal Nicotine exposure: A review. Intern. J. Dev. Neurosci. [Internet]. 2022; 82: 3–18. doi: https://doi.org/kmnd

Mahmoudzadeh L, Froushani SMA, Ajami M, Mahmoudzadeh M. Effect of Nicotine on immune system function. Adv. Pharm. Bull. [Internet]. 2023; 3:69–78. doi: https://doi.org/kmnf

White HK, Levin ED. Four–week Nicotine skin patch treatment effects on cognitive performance in Alzheimer’s disease. Psychopharmacol. [Internet]. 1999; 143:158–165. doi: https://doi.org/cfjzpp

Hsieh MT, Tseng PT, Wu YC, Tu YK, Wu HC, Hsu CW, Lei WT, Stubbs B, Carvalho AF, Liang CS, Yeh TW, Chen TY, Chu CS, Li JC, Yu CL, Chen YW, Li DJ. Effects of different pharmacologic smoking cessation treatments on body weight changes and success rates in patients with Nicotine dependence: A network meta–analysis. Obes. Rev. [Internet]. 2019; 20:895–905. doi: https://doi.org/kmng

Stefan MS, Pack Q, Shieh MS, Pekow PS, Bernstein SL, Raghunathan K, Nason KS, Lindenauer PK. The Association of Nicotine replacement therapy with outcomes among smokers hospitalized for a major surgical procedure. Chest. [Internet]. 2020; 157:1354–1361. doi: https://doi.org/kmnh

Borrego–Soto G, Eberhart JK. Embryonic Nicotine exposure disrupts adultsocial behavior and craniofacial development in zebrafish. Toxics. [Internet]. 2022; 10:612 doi: https://doi.org/kmnj

Dean R, Duperreault E, Newton D, Krook J, Ingraham E, Gallup J, Franczak BC, Hamilton TJ. Opposing effects of acute and repeated Nicotine exposure on boldness in Zebrafish. Scientif. Rep.. [Internet]. 2020; 10:8570 doi: https://doi.org/gnmsvb

Klee EW, Ebberh JO, Schneider H, Hurt RD, Ekker AC. Zebrafish for the study of the biological effects of Nicotine. Nicotine Tob. Res. [Internet]. 2011; 13(5):201–312. doi: https://doi.org/fwzpqp

Victoria S, Hein M, Harrahy E, King–Heiden TC. Potency matters: Impacts of embryonic exposure to nAChR agonists thiamethoxam and Nicotine on hatching success, growth, and neurobehavior in larval zebrafish. J. Toxicol. Environ. Health. Part A. [Internet]. 2022; 85:767–782. doi: https://doi.org/gqs343

Bhattacharya B, Narain V, Bondesson M. E–cigarette vaping liquids and flavoring chemical cinnnamaldehyde perturb bone cartilage and vascular development in Zebrafish embryos. Aquat. Toxicol. [Internet]. 2021; 240:105995. doi: https://doi.org/kmnk

Svoboda KR, Vijayaraghavan S, Tanguay RL. Nicotinic receptors mediate changes in spinal motoneuron development and axonal pathfinding in embryonic Zebrafish exposed to Nicotine. J. Neurosci. [Internet]. 2002; 22(24):10731–10741. doi: https://doi.org/gp6zbq

Çek Ş, Shang M, Perera DA, Baofeng S, Dunham R. Fish stem cells: classification, resources, characteristics and application areas. J. Limnol. Freshw. Fisheries Res. [Internet]. 2016; 2:107–119. doi: https://doi.org/kmnm

Dede K, Çek–Yalnız Ş. Artificial reproduction of zebrafish Danio rerio under controlled laboratory condition. Asian J. Fish Aquat. Res. [Internet]. 2018; 2:1–8. doi: https://doi.org/kmnn

Howe K, Clark, MD, Torroja CF, Torrance J, Berthelo C. The zebrafish reference genome sequence and its relationship to the human genome. Nature. [Internet]. 2013; 496:498–503. doi: https://doi.org/k9c

Parker B, Connaughton VP. Effects of Nicotine on growth and development in larval zebrafish. Zebrafish. [Internet]. 2007; 4:59–68. doi: https://doi.org/csgzb8

Kord MI, Maulu S, Srour TM, Omar EA, Farag AA, Nour AAM, Hasimuna OJ, Abdel–Tawwab M, Khalil HS. Impacts of water additives on water quality, production efficiency, intestinal morphology, gut microbiota, and immunological responses of Nile tilapia fingerlings under a zero water exchange system. Aquacult. [Internet]. 2022; 547:737503. doi: https://doi.org/kmnp

Zar JH. Statistical significance of mutation frequencies, and the power of statistical testing, using the Poisson distribution. Biom. J. [Internet]. 1984; 26:83–88. doi: https://doi.org/fr4rw8

Matta SG, Balfour DJ, Benowitz NL, Boyd RT, Buccafusco J, Caggiula AR, Craig CR, Collins AC, Damaj MI, Donny EC, Gardiner PS, Grady SR, Heberlein U, Leonard SS, Levin ED, Lukas RJ, Markou A, Marks MJ, McCallum SE, Parameswaran N, Perkins KA, Picciotto MR, Quik M, Rose JE, Rothenfluh A, Schafer WR, Stolerman IP, Tyndale RF, Wehner JM, Zirger JM. Guidelines on Nicotine dose selection for in vivo research. Psychopharmacol. [Internet]. 2007; 190:269–319. doi: https://doi.org/d26xt5

Jonz MG, Zachar PC, Da Fonte DF, Mierzwa AS. Peripheral chemoreceptors in fish: A brief history and look ahead. Comp. Biochem. Physiol. [Internet]. 2015; 186:27–38. doi: https://doi.org/f7j3qv