Intermediate hosts with genetic resistance to Fasciola hepatica and preliminary evaluation of its use in the control of fasciolosis in cattle in the Cajamarca Area, Perú

• Marco Cabrera–González Instituto Nacional de Innovación Agraria (INIA), Dirección de Desarrollo Tecnológico Agrario, Estación Experimental Baños del Inca. Baños del Inca, Cajamarca, Perú
• Cristian Hobán Universidad Nacional de Cajamarca, Facultad de Ciencias Veterinarias, Laboratorio de Inmunología. Cajamarca, Perú
• Carlos Quilcate–Pairazamán Instituto Nacional de Innovación Agraria (INIA), Dirección de Desarrollo Tecnológico Agrario. La Molina, Lima, Perú
• Medali Cueva–Rodríguez Instituto Nacional de Innovación Agraria (INIA), Dirección de Desarrollo Tecnológico Agrario, Estación Experimental Baños del Inca. Baños del Inca, Cajamarca, Perú

DOI: https://doi.org/10.52973/rcfcv-e34266

Keywords: Fasciola hepatica, cattle, resistance, Lymnaea viatrix

Abstract

With the objective of preliminarily evaluating populations of morphotypes with patterns of phenotypic markers of resistance to artificial infection by miracidium for their use in the control of fasciolosis in cattle, intermediate hosts with resistance to infective forms of Fasciola hepatica were multiplied under conditions laboratory, 850 snails were collected from the ditches and swampy areas of 8 herds located in the districts of La Encañada and Baños del Inca, Peru, located at an altitude of 2,626 to 3,115 meters above sea level (msnm), of the genus Lymnaea viatrix, selecting 400 snails with patterns of susceptibility/resistance to artificial infection by miracidium (shell morphometry, oviposition behavior, mantle pigmentation pattern). Two breeding methods of multiplication of morphotypes were tested, observing that breeding method 1 obtained a lower average mortality of n=11 ± 2.44 in relation to method 2, in terms of fecundity it was higher in method 1 (n=8) in relation to method 2 (n=3); The viability of the egg masses in method 1 was 60% and in method 2 was 48.4%. The incubation time of the eggs was 19 days at 18°C. The resistance found and observed of these morphotypes evaluated the preliminary resistance against miracidium in laboratory conditions, observing that it was 5% in the genus Lymnaea and can be extrapolated to field conditions, taking into account the observations made regarding adaptability, population density, adaptability and survival during the time of year and the parasite load can be used as an alternative control to the inadequate chemical control that causes the resistance phenomenon of F. hepatica.

References

Solana MV, Mera–Sierra R, Scarcella S, Neira G, Solana HD. In vivo assessment of closantel ovicidal activity in Fasciola hepatica eggs. Experim. Parasitol. [Internet]. 2016; 160:49–53. doi: https://doi.org/k4jm

Beesley NJ, Caminade C, Charlier J , Flynn RJ, Hodgkinson JE., Martinez–Moreno A, Martinez–Valladares M, Perez J, Rinaldi L, Williams DJL. Fasciola and fasciolosis in ruminants in Europe: Identifying research needs. Transb. Emerg. Dis. [Internet]. 2018; 65:199–216. doi: https://doi.org/gb2qgd

Cwiklinski K, O’Neill SM, Donnelly S, Dalton JPA. Prospective view of animal and human Fasciolosis. Parasite Immunol. [Internet]. 2016; 38(9):558–568. doi: https://doi.org/gqc57n

Zerna G, Spithill TW, Beddoe T. Current Status for Controlling the Overlooked Caprine Fasciolosis. Anim. [Internet]. 2021; 11(6):1819. doi: https://doi.org/k4jn

Palacio–Collado D, Bertot–Valdés JA, Beltrao–Molento M. Fasciolosis en Cuba y el mundo. Rev. Prod. Anim. [Internet] 2020 [Consultado 20 May 2023]; 32(3):e3658. Disponible en: https://bit.ly/3Qr5kvA

Hodgkinson JE, Cwiklinski K, Beesley N, Hartley C, Allen K, Williams DJL. Clonal amplification of Fasciola hepatica in Galba truncatula: Within and between isolate variation of triclabendazole–susceptible and–resistant clones. Parasit. Vectors. [Internet]. 2018; 11(1):1–9. doi: https://doi.org/k4j7

Amanda CV, Lilian SR, Carlos AD, Francisco SA. Resistance to anthelmintics and prevalence of bovine fasciolosis in dairy farms in jauja, Peru. Rev. Investig. Vet. Peru. 2012; 23(1):90–97.

Beesley NJ, Cwiklinski K, Allen K, Hoyle RC, Spithill TW, James La Course E, Williams DJL, Paterson S, Hodgkinson JE. A major locus confers triclabendazole resistance in Fasciola hepatica and shows dominant inheritance. PLoS Pathog. [Internet]. 2023; 19(1):1–28. doi: https://doi.org/k4j8

López–Villacís IC, Artieda–Rojas JR, Mera–Andrade RI, Muñoz–Espinoza MS, Rivera–Guerra VE, Cuadrado–Guevara AC, Zurita–Vásquez JH, Montero–Recalde MA. Fasciola hepática: aspectos relevantes en la salud animal. J. the Selva Andina Anim. Sci. [Internet]. 2017; 4(2):137–146. doi: https://doi.org/k4j9

Brockwell YM, Elliott TP, Anderson GR, Stanton R, Spithill TW, Sangster NC. Confirmation of Fasciola hepatica resistant to triclabendazole in naturally infected Australian beef and dairy cattle. Intern. J. Parasitol: Drugs Drug Resist. [Internet]. 2021; 4(1):48–54. doi: https://doi.org/htj8

Hickman Jr. CP, Roberts LS, Hickman FM. Zoología Principios Integrales. México: Editorial Interamericana. 1986; p. 204–237.

Urquhart GM, Armour J, Duncan JC, Dunn A.M. Parasitología Veterinaria. Zaragoza, España: Editorial Acribia. 2001; p. 116–127

Soulsby EJ. Parasitología y Enfermedades en los animales domésticos. México: Editorial Interamericana. 1987; 823 p.

Alba A, Vázquez AA, Sánchez J, Lounnas M, Pointier JP, Hurtrez–Boussès S, Gourbal, B. Patterns of distribution, population genetics and ecological requirements of field–occurring resistant and susceptible Pseudosuccinea columella snails to Fasciola hepatica in Cuba. Sci. Rep. [Internet]. 2019; 9(1):14359. doi: https://doi.org/k4kb

Alba A, Tetrau G, Chaparro C, Sánchez J, Vázquez AA, Gourbal B. Natural resistance to Fasciola hepatica (Trematoda) in Pseudosuccinea columella snails: A review from literature and insights from comparative “omic” analyses. Dev. Comp. Immunol. [Internet]. 2019; 101:103463. doi: https://doi.org/gmx48b

De NV, Le TH, Agramunt VH, Mas–Coma S. Early Postnatal and Preschool–Age Infection by Fasciola spp.: Report of Five Cases from Vietnam and Worldwide Review. Ame. J. Trop. Med. Hyg. [Internet]. 2020; 103(4):1578–1589. doi: https://doi.org/ghxpc9

Dreyfuss G, Rondelaud D, Vareille–Morel C. Oviposition of Lymnaea truncatula infected by Fasciola hepatica under experimental conditions. Parasitol. Res. 1999; 85:589–593

Bardales–Valdivia JN. Caracterización molecular y estudio ecológico del entorno de los moluscos hospederos intermediarios de Fasciola hepatica en las provincias de Cajamarca, San Marcos y Cajabamba – Perú. [tesis doctoral en Internet]. Perú: Universidad Nacional de Cajamarca; 2017 [consultado 25 May 2023]; 135 p. Disponible en: https://bit.ly/3QTCh56.

Statistical Analysis System Institute (SAS). User’s Guide Statistic. Release 9.4. Cary, NC, USA: SAS Institute. 2013; 646 p.

Pereira De SC, Magalhaes KG: Rearing of Lymnaea columella (Say, 1817), intermediate host of Fasciola hepatica (Linnaeus, 1758). Mem. Inst. Oswaldo Cruz. [Internet]. 2000; 95(5):739–741. doi: https://doi.org/d8vnws

Vallena R. Prevalencia de fasciolosis en animales beneficiados en el camal municipal de Cajamarca. [tesis de grado]. Perú: Universidad Nacional de Cajamarca; 1986; 70 p.

Díaz E, Rojas J. Helmintosis que causan pérdidas económicas por decomisos en animales beneficiados en el camal de Cajamarca. En: Libro Resumen XXVII Reunión científica APPA. 20–24 de septiembre de 2004, Piura, Perú. 2004; p. 150.

Cuadros–Medina S, Manrique–Meza J. Fasciolosis: Buscando Estrategias de Control. Arequipa, Perú: Editorial Universidad Católica de Santa María. 2002; 126 p.

Ministerio de Salud. Anales de Seminario Internacional de Zoonosis y Enfermedades de Transmisión Alimentaria. Programa Nacional de Zoonosis. Ministerio de Salud. Lima, Perú. 1989; 90 p.

Lapage G. Parasitología Veterinaria. México: Editorial Continental. 1983; 790 p.

Vilca F. Fasciolosis en bovinos beneficiados en el camal municipal de Puno mediante dos métodos de diagnóstico. Puno, Perú: Oficina Unidad de Investigación, UNA. 2000; 3 p.