Functional morphology of arteriovenous anastomoses on rat ovary

• Kaan Çimen Sivas Cumhuriyet University, Faculty of Medicine, Department of Anatomy. Sivas, Türkiye
• Mehmet Çimen Sivas Cumhuriyet University, Faculty of Medicine, Department of Anatomy. Sivas, Türkiye

DOI: https://doi.org/10.52973/rcfcv-e34303

Keywords: Arteriovenous anastomoses, rat ovary, electron microscopy, functional morphology

Abstract

This study aims to show the morphology of the structure involved in the function of arteriovenous anastomoses (AVA) under electron microscope. The study used 20 adult females of Rattus norvegicus. Ovarian tissue samples were fixed in 3% Glutaraldehyde in phosphate buffer, and then post–fixed in increasing concentrations of Ethanol, tissues were embedded in Epon resin. Semi–thin tissue sections were double stained with Uranyl acetate saturated in 70% Ethanol and lead Citrate. The ultrathin sections were examined in a JEOL 100 C electron microscope. In the opened AVA section, tunica intima, tunica media, and adventitia layers were observed in the vessel wall structure. The endothelial cell was present in the tunica intima, and the lumen was open. Thick layered smooth muscle cells were found in the tunica media. The muscles were arranged inner longitudinally and outer circularly. The internal elastic membrane lies between the circular and longitudinal smooth muscle. In another section taken from the tunica adventitia, fibroblasts were observed between dense elastic and collagen fibrils. The longitudinal smooth muscle was contracted in the closed AVA section, and the lumen appeared in the typical asterisks shape. This study showed the functional morphology of the AVA's and detailed vessel wall structures in the rat ovary. Lumen structure with open and closed AVAs is also shown. With observations from this study, the functional properties of the formations in the AVA wall structure are explained in the rat ovary.

References

Clara M. Die Arterio–Venösen Anastomosen: Anatomie, Biologie, Pathologie. [Internet]. 2nd ed. Vienna: Springer. 1956; 315 p. doi: https://doi.org/k48f

Sherman JL. Normal arteriovenous anastomoses. Med. [Internet]. 1963; 42(4):247–267. doi: https://doi.org/ffn7ds

Clark ER. Arterio–venous anastomoses. Physiol. Rev. [Internet]. 1938; 18(2):229–247. doi: https://doi.org/k48h

Kern MD, Coruzzi L. The structure of the canary's incubation patch. J. Morph. [Internet]. 1979; 162(3):425–452. doi: https://doi.org/fhhbdz

Gorgas M, Böck P, Tischendorf F, Curri SB. The fine structure of human digital arteriovenous anastomoses (Hoyer–Grosser's organs). Anat. Embryol. [Internet]. 1977; 150:269–289. doi: https://doi.org/b9dnrv

Grant RT. Observations on direct communications between arteries and veins in the rabbit's ear. Heart J. Stud. Circ. 1930; 15(3):281–303.

Clark ER, Clark EL. Observations on living arteriovenous anastomoses as seen in transparent chambers introduced into the rabbit's ear. Ame. J. Anat. [Internet]. 1934; 54(2):229–286. doi: https://doi.org/c4fscp

Walløe L. Arterio–venous anastomoses in the human skin and their role in temperature control. Temperat. [Internet]. 2016; 3(1):92–103. doi: https://doi.org/k48j

Mescon H, Hurley Jr. HJ, Moretti G. The anatomy and histochemistry of the arteriovenous anastomosis in human digital skin. J. Invest. Dermatol. [Internet]. 1956; 27(3):133–145. doi: https://doi.org/bjjk6j

Wu F, Shen X, Eberlin KR, Sun Z, Zhou X, Xue M. The use of arteriovenous anastomosis for venous drainage during Tamai zone I fingertip replantation. Injury. [Internet]. 2018; 49(6):1113–1118. doi: https://doi.org/gdr8gw

Lipa M, Kosinski P, Stanirowski P, Wielgos M, Bomba–Opon D. Vascular anastomoses in intrauterine growth in monochorionic twins. J. Perinat. Med. [Internet]. 2020; 48(6):539–543. doi: https://doi.org/gndchc

Grinsell D, Rajkomar AK, Rozen WM, Ramsey KW. Refining our knowledge of macrovascular arteriovenous shunts (MAS): Anatomical and pathological studies. J. Plast. Reconstr. Aesthet. Surg. [Internet]. 2020; 73(8): 1490–1498. doi: https://doi.org/k48m

Krmpotic CM, Loza CM, Negrete J, Scarano AC, Carlini AA, Guerrero A, Barbeito CG. Integument in Antarctic seals: a comparative study and its relation to extreme environments. Acta Zool. [Internet]. 2018; 99(3):281–295. doi: https://doi.org/gdqmpg

Hussein MM. Structural and functional characteristics of the special regulatory devices in the peripheral pulmonary circulation in rabbits. Protoplasma. [Internet]. 2020; 257(3):755–766. doi: https://doi.org/k48q

Miller SL, Dickson K, Jenkin G, Walker DW. Physiological evidence for arteriovenous anastomoses in the uterine circulation of late‐pregnant ewes. Clin. Exp. Pharmacol. Physiol. [Internet]. 1998; 25(2):92–98. doi: https://doi.org/dp35m9

Nio–Kobayashi J, Miyazaki K, Hashiba K, Okuda K, Iwanaga T. Histological analysis of arteriovenous anastomosis–like vessels established in the corpus luteum of cows during luteolysis. J. Ovarian Res. [Internet]. 2016; 9(67)1–14. doi: https://doi.org/k48r

Mokhtar DM. Characterization of the fish ovarian stroma during the spawning season: Cytochemical, immunohistochemical and ultrastructural studies. Fish Shellfish Immunol. [Internet]. 2019; 94:566–579. doi: https://doi.org/k5c4

Mokhtar DM, Abd‐Elhafez EA. Morphological studies on the peripheral circulation of the ovary in one‐humped camel (Camelus dromedarius). Anat. Histol. Embryol. [Internet]. 2016; 45(4):319–328. doi: https://doi.org/f8vdfr

Lijima T, Hasegawa K, Hirose H. Wall structure of arteriovenous anastomoses in the rabbit ear. Cell Tissue Res. [Internet]. 1988; 252:1–8. doi: https://doi.org/dj2s7n

Midtgård U. Arteriovenous anastomoses in the incubation patch of herring gulls. The Condor. [Internet]. 1985; 87(4):549–551. doi: https://doi.org/ddkqbb

Çimen M. Development of arteriovenous anastomoses in the skins of rats during the fetal period. Rev. Cientific. FCV–LUZ. [Internet]. 2003 [cited 15 Jun 2023]; 13(6):480–483. Available in: https://bit.ly/47Au5wb.

Çimen M, Gürsoy E, Kaloglu C. Various types of glomus anastomoses in developing rat fetuses. Anat. Histol. Embryol. [Internet] 2000; 29(1):45–50. doi: https://doi.org/bmfrvw

Çimen M. Arteriovenous anastomosis ultrastructure in the rat ovary. Indian J. Anim. Sci. [Internet]. 2003 [cited 18 May 2023]; 73(6):630–632. Available in: https://bit.ly/40O9MJK.