Evaluating Adropine levels in kidney tissue after Methotrexate treatment in rats: a prospective experimental study

• Karakeci Ahmet Firat University, School of Medicine, Department of Urology. Elazig, Türkiye
• Kuloglu Tuncay Firat University, School of Medicine, Department of Histology and Embryology. Elazig, Türkiye
• Acisu Tutku Can Firat University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination. Elazig, Türkiye
• Keles Ahmet Istanbul Medeniyet University, School of Medicine, Department of Urology. Istanbul, Türkiye
• Ozan Tunc Firat University, School of Medicine, Department of Urology. Elazig, Türkiye
• Vural Osman Firat University, School of Medicine, Department of Histology and Embryology. Elazig, Türkiye
• Orhan Irfan Firat University, School of Medicine, Department of Urology. Elazig, Türkiye
• Sabaz Karakeci Emel Health Sciences University, Elazig Fethi Sekin City Hospital, Department of Physical Therapy and Rehabilitation. Elazig, Türkiye

DOI: https://doi.org/10.52973/rcfcv-e34323

Keywords: Antioxidants, adropin protein, kidney diseases, Methotrexate, oxidative stress

Abstract

In this study, it was aimed to investigate Adropin levels in kidney tissues after Methotrexate (MTX) administration to identify potential changes following administration of agents with antioxidant/anti–inflammatory potential. Twenty four adult rats male albino Wistar rats were used in this study, and randomly divided into four groups. Control: These rats did not receive any treatment during the 14–day (d) experiment. N–acetylcysteine (NAC): These rats were administered 100 mg·kg^-1·day^-1 NAC intraperitoneally (i.p.) for 14 d. MTX: A single dose of 20 mg·kg^-1 MTX was administered i.p. at the beginning of the study. MTX+ NAC: A single dose of 20 mg·kg^-1 MTX was administered i.p. at the beginning of the study, and the rats were given 100 mg·kg^-1·day^-1 NAC i.p. for 14 d. Total antioxidant, and serum Adropin levels were found to be the lowest in the MTX group while the oxidant levels were significantly lower in the MTX group than in the MTX+NAC group (P<0.001). TUNEL positivity was similar among the groups, and no significant differences were observed. It was considered that these findings have shed light on the role of Adropin in the development of kidney failure following MTX administration.

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