Los efectos del tratamiento solo y combinado de extracto alcohólico de Tarántula cubensis y enantato de metenolona en dos huesos largos de ratas jóvenes

• Mustafa Sedat Arslan Selcuk University, Faculty of Veterinary Medicine, Department of Anatomy. Konya, Turkey
• Kamil Besoluk Selcuk University, Faculty of Veterinary Medicine, Department of Anatomy. Konya, Turkey

DOI: https://doi.org/10.52973/rcfcv-e33274

Palabras clave: Extracto alcohólico, fémur, húmero, fémur, húmero, enantato de metenolona, rata, Tarantula cubensis

Resumen

El objetivo de este estudio fue determinar los efectos del tratamiento solo y combinado de extracto alcohólico de Tarantula cubensis y enantato de metenolona (EM) sobre parámetros morfométricos de húmero y fémur en ratas jóvenes. En este estudio, se utilizaron 36 ratas Wistar Albino de cinco semanas de edad. Los animales se dividieron en 4 grupos; Grupo control (n:6, solución salina normal, 0,2 mL·rata^-1, por vía subcutánea –SC–), grupo TCAE (extracto alcohólico de Tarantula cubensis, 0,2 mL·rata^-1, por vía SC), grupo ME (enantato de metenolona, 10 mg·kg^-1, por vía intramuscular –IM–) y TCAE + grupo ME (extracto alcohólico de Tarantula cubensis, 0,2 mL·rata^-1, por vía SC + enantato de metenolona, 10 mg·kg^-1, por vía IM). Los tratamientos farmacológicos se realizaron una vez por semana durante 7 semanas. Al final del experimento, todos los animales fueron sacrificados y sus huesos húmero y fémur fueron resecados y se determinaron sus parámetros morfométricos. No se determinaron diferencias estadísticas (P>0,05) entre los grupos en cuanto a los puntos de referencia anatómicos (longitud, grosor del cuerpo, diámetro del cavum medular y grosor de la corteza) de los huesos húmero y fémur. En conclusión, se encontró que los tratamientos solos y combinados de extracto alcohólico de Tarantula cubensis y EM (a dosis normal) no tuvieron efectos sobre los parámetros morfométricos de los huesos del húmero y fémur en ratas en el período de crecimiento. Sin embargo, es posible que estos efectos no se observen con dosis altas y tratamientos a largo plazo en ratas.

Citas

Coşkun D. Veteriner Destek Tedavi: Tarantula Cubensis Alkolik Ekstraktı, İnaktif Parapoxvirüs Ovis ve Corynebacterium Cutis Lizatı. Dicle Üniversitesi Veteriner Fakültesi Dergisi. [Internet]. 2017 [cited 24 April 2023]; 10(1):30–37. Available in: https://bit.ly/45AgxzX.

Canbar R, Akcakavak G, Uslu M, Bas AL. Determination of embryotoxic effects of Tarantula cubensis alcoholic extract with in ovo model. Magy. Allatorvosok Lapja. [Internet]. 2021 [cited 18 April 2023]; 143(8):497–504. Available in: https://bit.ly/47ElXM4.

Albay MK, Sahinduran S, Kale M, Karakurum MC, Sezer K. Influence of Tarantula cubensis extract on the treatment of the oral lesions in cattle with blue tongue disease. Kafkas Univ. Vet. Fak. Derg. [Internet]. 16(4):593–96. doi: https://doi.org/kp7n

Bonamin LV, Cardoso TN, de Carvalho AC, Amaral JG. The use of animal models in homeopathic research – a review of 2010–2014, PubMed indexed papers, Homeopathy. [Internet]. 2015; 104 (04):283–91. doi: https://doi.org/f744t2

Dik B, Er A, Corum O. Effect of alcoholic extract of Tarantula cubensis (Theranekron®) on serum thiobarbituric acid–reactive species concentrations in sheep. Eurasian J. Vet. Sci. [Internet]. 2014 [cited 18 April 2023]; 30 (2):68–71. Available in: https://bit.ly/45y3e31.

Er A, Ozdemir O, Coskun D, Dik B, Bahcivan E, Faki HE, Yazar E. Effects of Tarantula cubensis alcoholic extract and Nerium oleander distillate on experimentally induced colon cancer. Rev. Med. Vet. [Internet]. 2019 [cited 18 April 2023]; 170 (1–3):15–21. Available in: https://bit.ly/3P1rllc.

Akcakavak G, Ozdemır O. Effect of Tarantula cubensis alcoholic extract on tumour pathways in azoxymethane–induced colorectal cancer in rats. Acta Vet. Brno. [Internet]. 2023; 92(1):79–88. doi: https://doi.org/kp7q

Özdemir Ö, Akçakavak G, Tuzcu M. Effect of Tarantula cubensis alcoholic extract and Nerium oleander distillate on cell proliferation markers in colon carcinogenesis. Rev. Cientif. FCV–LUZ. [Internet]. 2022; 32(1):1–8. doi:10.52973/rcfcv–e32150.

Kacar C, Kaya S. Uterine Infections in cows and effect on reproductive performance. Kafkas Univ. Vet. Fak. Derg. [Internet]. 2014; 20(6):975–82. doi: https://doi.org/kp7r

Sardari K, Mohri M, Sabzevari S, Fathi B. Effects of the Theranekron® an alcoholic extract of the Tarantula cubensis on hematology and serum biochemical properties in horses. Iran. J. Vet. Sci. Technol. [Internet]. 2014; 3(2):9–16. doi: https://doi.org/kp7s

Gok R, Yalcin AD, Tural U. Abuse of Anabolic Steroids–Review. Bagim. Derg. [Internet]. 2016 [cited 11 May 2023]; 17(4):172–80. Available in: https://bit.ly/3KSk44D.

Mycek MJ, Harvey RA, Champe PC. Farmakoloji. 2nd ed. Ankara (Turkey): Günes Kitapevi; 2001. p 270–1.

Kayaalp SO. Rasyonel Tedavi Yonunden Tibbi Farmakoloji. 11th ed. Ankara (Turkey): Feryal Matbaasi; 2005. p 1126–57.

Bokesoy TA, Cakici I, Melli M. Farmakoloji Ders Kitabi. Ankara (Turkey): Gazi Kitabevi; 2000. p 380–5.

Guyton AC, Hall JE. Textbook of Medical Physiology. 9th ed. Philadelphia (PA): WB Saunders Company; 2001. p 1011–3.

Koc H, Yuksel O. Kadinlarda fiziksel ve fizyolojik performansin degerlendirilmesi. Dumlupinar Uni. Sos. Bil. Der. [Internet]. 2007 [cited 11 May 2023]; 9:1–12. Available in: https://bit.ly/3qGiE6A.

Maravelias C, Dona A, Stefanidou M, Spiliopoulou C. Adverse effects of anabolic steroids in athletes: A constant threat. Toxicol. Lett. [Internet]. 2005; 158(3):167–75. doi: https://doi.org/fth3pp

Dursun N. Veteriner Anatomi. Ankara (Turkey): Medisan Yayinlari; 2000. p 67–85.

Goldfarb AF, Napp EE, Stone ML, Zuckerman MB, Simon J. The anabolic effects of norethandrolone, a 19–nortestosterone derivative. Obstet. Gynecol. 1958; 11(4):454–8. PMID: 13517756.

Tauchen J, Jurasek M, Huml L, Rimpelova S. Medicinal use of testosterone and related steroids revisited. Molecules. [Internet]. 2021; 26(4):1032. doi: https://doi.org/gjpmn7

Ozdemir M, Lok S. The effects of methenolone enanthate supplement with exercise on rats’ bones. Turkish J. Sport Exercise. [Internet]. 2019; 21(2): 276–80. doi: https://doi.org/kp7v

Bozkurt I, Pepe K, Ozdemir M, Ozdemir O, Coskun A. Morphometric evaluation of the effect of methenolone enanthate on femoral development in adolescent rats. Sci. Res. Essays. [Internet]. 2011 [cited 11 May 2023]; 6(7): 1634–8. Available in: https://bit.ly/3QGWagg.

Lok S, Yalcin H. Morphometric effect of nandrolone decanoate used as doping in sport on femur of rats in puberty period. Arch. Budo. [Internet]. 2010 [cited 11 May 2023]; 6:218–220. Available in: https://bit.ly/3E4eoAI.

Ozdemir M, Yalcın H. The morphometric effects of testosterone, used as a doping agent, on the humerus and femur of pubescent male and female rats. Selcuk Univ. Beden Egit. Spor. Bilim. Derg. [Internet]. 2011; 13(2):172–7. doi: https://doi.org/kp7x

Bozkurt I, Ozdemir M, Pepe K, Ozdemir O, Coskun A. Morphometric evaluation of the effect of methenolone enanthate on humeral development in adolescent rats. Sci. Res. Essays. [Internet]. 2011 [cited 11 May 2023]; 6(13):2676–81. Available in: https://bit.ly/47IcNOP.

Lok S, Yalcin H. Morphometric Effect of Nandrolone on Humerus of the Pubertal Term Rats. J. Anim. Vet. Adv. [Internet]. 2010; 9:1771–1775. doi: https://doi.org/dmrrhm

Nomina Anatomica Veterinaria. International committee on veterinary gross anatomical nomenclature. 6th ed. Editorial Committee Hanover (Germany), Ghent (Belgium), Columbia, MO (U.S.A.), Rio de Janeiro (Brazil): 2017; p 11–29.

Quaglio G, Fornasiero A, Mezzelani P, Moreschini S, Lugoboni F, Lechi A. Anabolic steroids: dependence and complications of chronic use. Intern Emerg. Med. [Internet]. 2009; 4(4):289–96. doi: https://doi.org/btzx4f

Ozdemir M, Yalcın H. The effects of testosterone, a prohibited substance, on the body and organ weights of pubescent rats. Ovidius Univ. Ann. Ser. Phys. Educ. Sport. Sci. Mov. Health. [Internet]. 2012 [cited 15 May 2023]; 12(1):83–8. Available in: https://bit.ly/3QNdOPt.

Bhasin S, Storer TW, Berman N, Callegari C, Clevenger B, Phillips J, Bunnell T, Tricker R, Shirazi A, Casaburi R. The effects of supraphysiologic doses of testosterone on muscle size and strength in normal men. N. Engl. J. Med. 1996; 335:1–7. [Internet]. doi: https://doi.org/d3678s