Prevención de úlceras estomacales inducidas por etanol en ratas utilizando extracto de hoja de Senecio perralderianus

• Walid Mamache University of Setif, Faculty of Natural and Life Sciences, Department of Biochemistry, Laboratory of applied phytotherapy to chronic diseases. Setif, Algeria
• Hassiba Benabdallah University of Setif, Faculty of Natural and Life Sciences, Department of physiology and animal biology, Laboratory of applied phytotherapy to chronic diseases. Setif, Algeria
• Ahlem Hannachi University of Setif, Faculty of Natural and Life Sciences, Department of Biochemistry, Laboratory of applied phytotherapy to chronic diseases. Setif, Algeria
• Amel Boukabes University of Setif, Faculty of Natural and Life Sciences, Department of Biochemistry, Laboratory of applied phytotherapy to chronic diseases. Setif, Algeria
• Amor Bencheikh University of Setif, Faculty of Natural and Life Sciences, Department of physiology and animal biology, Laboratory of applied microbiology. Setif, Algeria
• Abderrahim Benslama University of Msila, Faculty of Sciences, Department of Biochemistry and Microbiology. Msila, Algeria
• Hind Amira University of Setif, Faculty of Natural and Life Sciences, Department of physiology and animal biology, Laboratory of applied phytotherapy to chronic diseases. Setif, Algeria
• Fatima Bencheikh University of Setif, Faculty of Natural and Life Sciences, Department of physiology and animal biology, Laboratory of applied phytotherapy to chronic diseases. Setif, Algeria
• Smain Amira University of Setif, Faculty of Natural and Life Sciences, Department of physiology and animal biology, Laboratory of applied phytotherapy to chronic diseases. Setif, Algeria

DOI: https://doi.org/10.52973/rcfcv-e34348

Palabras clave: Senecio perralderianus, extracto de metanol, úlcera gástrica, estrés oxidativo

Resumen

Senecio perralderianus pertenece a la familia Asteraceae y se encuentra únicamente en Argelia. Algunas especies de esta familia se utilizan para curar problemas gastrointestinales en la medicina convencional por sus propiedades antioxidantes y antiinflamatorias. Esta investigación se llevó a cabo para determinar si el extracto metanólico de las hojas de S. perralderianus tenía efectos protectores sobre la gastroenteritis causada por el consumo de alcohol. Las Wistar ratas fueron ingeridas por vía oral con etanol al 100 % por vía oral para inducir úlcera gástrica y fueron pretratadas con 50; 100 y 200 mg·kg^-1 del extracto, además de 5 mg·kg^-1 de Ranitidina como fármaco de referencia positivo. El extracto había demostrado un efecto positivo para proteger las úlceras gástricas inducidas por el etanol con un porcentaje de protección del 71 al 88 %. Además, el pretratamiento de ratas aumentó significativamente los niveles de GSH, CAT y SOD in vivo como antioxidantes no enzimáticos y enzimáticas, y también redujo el nivel de peroxidación de lípidos. Las secciones histopatológicas, que mostraron la acción del extracto terapéutico, confirmaron sustancialmente estos hallazgos sobre la reducción de la zona de inflamación y la disminución de filtración de células inmunes causada por la toxicidad del etanol con dosis de extractos aumentadas en comparación con la Ranitidina. La actividad antiúlcera se debe a la inhibición del estrés oxidativo y la gastritis. Se asocia con un total de polifenoles, flavonoides, clorofila (a, b), y carotenoides cantidades sustanciales.

Citas

Roy S. Clinical study of peptic ulcer disease. Asian J. Biomed. Pharm Sci. 2016; 6(53):41-43.

Dunlap JJ, Patterson S. Peptic ulcer disease. Gastroenterol. Nurs. [Internet]. 2019; 42(5):451-454. doi: https://doi.org/gq32

Serafim C, Araruna ME, Júnior EA, Diniz M, Hiruma-Lima C, Batista L. A Review of the Role of Flavonoids in Peptic Ulcer (2010-2020). Molec. [Internet]. 2020; 25(22):5431. doi: https://doi.org/gsch44

Yang H, Hu B. Immunological perspective: Helicobacter pylori infection and gastritis. Mediators Inflamm. [Internet]. 2022; 2022:2944156. doi: https://doi.org/mbkf

Sverdén E, Agréus L, Dunn JM, Lagergren J. Peptic ulcer disease. BMJ. [Internet]. 2019; 367:l5495. doi: https://doi.org/gh9dft

Amira S, Dade M, Schinella G, Ríos JL. Anti-inflammatory, anti-oxidant, and apoptotic activities of four plant species used in folk medicine in the Mediterranean basin. Pak. J. Pharm. Sci. 2012; 25(1):65-72. Cited in: PubMed; PMID 22186311.

Kebbi S, Noman L, Demirtas I, Bensouici C, Adem S, Benayache S, Benayache F, Seghiri R, Gok M. In vitro Antioxidant and Anticholinesterase Activities of Senecio massaicus Essential Oil and Its Molecular Docking Studies as a Potential Inhibitor of Covid-19 and Alzheimer’s Diseases. JBAPN. [Internet]. 2021; 11(4):380-394. doi: https://doi.org/mbkm

Lograda T, Ramdani M, Chalard P, Gharzouli R, Feguiredo G, Chalchat JC. Essential oil and antibacterial activity of Senecio perralderianus. Intern. J. Med. Aromat Plants. 2012; 2(4):632-637.

Bouchibane M, Zemouri M, Toumi R. [Contribution to the study of the vegetation of some mountains to the Kabylia of Babors (Northeastern Algeria)]. Bull. Soc. R. Sci. Liege. [Internet]. 2021; 90:317-360. French. doi: https://doi.org/mbkn

Mamache W, Amira S, Ben Souici C, Laouer H, Benchikh F. In vitro antioxidant, anticholinesterases, anti‐α‐amylase, and anti‐α‐glucosidase effects of Algerian Salvia aegyptiaca and Salvia verbenaca. J. Food Biochem. [Internet]. 2020; 44(11):e13472. doi: https://doi.org/mbkp

Gharzouli K, Gharzouli A, Amira S, Khennouf S. Prevention of ethanol-induced gastric lesions in rats by natural honey and glucose-fructose-sucrose-maltose mixture. Pharmacol. Res. [Internet]. 2001; 43(5):509. doi: https://doi.org/b3g8xb

Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem. [Internet]. 1949;177(2):751-766. doi: https://doi.org/gh4c93

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. [Internet]. 1979; 95(2):351-358. doi: https://doi.org/bktx4x

Ellman GL. Tissue sulfhydryl groups. Arch. Biochem. Biophys. [Internet]. 1959; 82(1):70-77. doi: https://doi.org/bz2vt8

Clairborne A. Catalase activity. In: Greenwald RA, editor. CRC Handbook of Methods for Oxygen Radical Research. Boca Raton, FL, USA: CRS Press. 1985. p. 283-284.

Gao R, Yuan Z, Zhao Z, Gao X. Mechanism of pyrogallol autoxidation and determination of superoxide dismutase enzyme activity. Bioelectrochem. Bioenerg. [Internet] 1998; 45(1):41-45. doi: https://doi.org/c7g5m8

Tête A, Gallais I, Imran M, Chevanne M, Liamin M, Sparfel L, Bucher S, Burel A, Podechard N, Appenzeller BMR, Fromenty B, Grova N, Sergent O, Lagadic-Gossmann D. Mechanisms involved in the death of steatotic WIF-B9 hepatocytes co-exposed to benzo[α]pyrene and ethanol: a possible key role for xenobiotic metabolism and nitric oxide. Free Radic Biol. Med. [Internet]. 2018; 129:323-337. doi: https://doi.org/gfqxkd

Hossen MA, Reza A, Ahmed AMA, Islam MK, Jahan I, Hossain R, Khan MF, Maruf MRA, Haque MA, Rahman MA. Pretreatment of Blumea lacera leaves ameliorate acute ulcer and oxidative stress in ethanol-induced Long-Evan rat: A combined experimental and chemico-biological interaction. Biomed. Pharmacother. [Internet]. 2021; 135:111211. doi: https://doi.org/mbkq

Toma W, Trigo JR, de Paula ACB, Brito ARMS. Preventive activity of pyrrolizidine alkaloids from Senecio brasiliensis (Asteraceae) on gastric and duodenal induced ulcer on mice and rats. J. Ethnopharmacol. [Internet]. 2004; 95(2-3):345-351. doi: https://doi.org/b3gw7j

Li WS, Lin SC, Chu CH, Chang YK, Zhang X, Lin CC, Tung YT. The Gastroprotective Effect of Naringenin against Ethanol-Induced Gastric Ulcers in Mice through Inhibiting Oxidative and Inflammatory Responses. Intern. J. Mol. Sci. [Internet]. 2021; 22(21):11985. doi: https://doi.org/gnprtc

Izhar H, Shabbir A, Shahzad M, Mobashar A, Ahmed SS. Phyllanthus reticulatus Prevents Ethanol-Induced Gastric Ulcer via Downregulation of IL-8 and TNF-α Levels. Evid Based Complement Alternat. Med. [Internet]. 2021; 2021:1734752. doi: https://doi.org/mbkt

Ababsa ZA, Kara Ali W, Abidli N, Akkal S, Medjroubi K. Chemical characterization and biological study of the species Senecio cineraria. World J. Environ. Biosci. [Internet]. 2018 [cited 24 Jul 2023]; 7(3):112-121. Available in: doi: https://bit.ly/3vpw4G0.

Cui J, Zong W, Zhao N, Yuan R. Burdock (Arctium lappa L.) leaf flavonoids rich in morin and quercetin 3-O-rhamnoside ameliorate lipopolysaccharide-induced inflammation and oxidative stress in RAW264.7 cells. Food Sci. Nutr. [Internet]. 2022; 10(8):2718-2726. doi: https://doi.org/mbkv

Cai SQ, Zhang Q, Zhao XH, Shi J. The In Vitro Anti-Inflammatory Activities of Galangin and Quercetin towards the LPS-Injured Rat Intestinal Epithelial (IEC-6) Cells as Affected by Heat Treatment. Molec. [Internet]. 2021; 26(24):7495. doi: https://doi.org/mbkw

Paciello F, Di Pino A, Rolesi R, Troiani D, Paludetti G, Grassi C, Fetoni AR. Anti-oxidant and anti-inflammatory effects of caffeic acid: in vivo evidences in a model of noise-induced hearing loss. Food Chem. Toxicol. [Internet]. 2020; 143:111555. doi: https://doi.org/mbkx

Hariprasath L, Raman J, Nanjian R. Gastroprotective effect of Senecio candicans DC on experimental ulcer models. J. Ethnopharmacol. [Internet]. 2012; 140(1):145-150. doi: https://doi.org/fxq2q3

Tata CM, Sewani-Rusike CR, Oyedeji OO, Mahlakata F, Shauli M, Nkeh-Chungag BN. Senecio serratuloides extract prevents the development of hypertension, oxidative stress and dyslipidemia in nitric oxide-deficient rats. J. Complement Integr. Med. [Internet]. 2020; 17(2):20180073. doi: https://doi.org/mbkz

Okoro O, Kadiri HE. Anti-Oxidant and Hepatoprotective Effects of Senecio biafrae on CCl 4-induced Liver Damage in Rats. Iranian J. Toxicol. [Internet]. 2019; 13(2):31-35. doi: https://doi.org/mbk2

Afifi SM, Ammar NM, Kamel R, Esatbeyoglu T, Hassan HA. β-Sitosterol Glucoside-Loaded Nanosystem Ameliorates Insulin Resistance and Oxidative Stress in Streptozotocin-Induced Diabetic Rats. Antioxidants (Basel). [Internet]. 2022; 11(5):1023. doi: https://doi.org/mbk3

Mohod SM, Kandhare AD, Bodhankar SL. Gastroprotective potential of Pentahydroxy flavone isolated from Madhuca indica J. F. Gmel. leaves against acetic acid-induced ulcer in rats: The role of oxido-inflammatory and prostaglandins markers. J. Ethnopharmacol. [Internet]. 2016; 182:150-159. doi: https://doi.org/f8g8qf

Liu Y, Gou L, Fu X, Li S, Lan N, Yin X. Protective effect of rutin against acute gastric mucosal lesions induced by ischemia-reperfusion. Pharm. Biol. [Internet]. 2013; 51(7):914-919. doi: https://doi.org/mbk4

Zhou D, Yang Q, Tian T, Chang Y, Li Y, Duan LR, Li H, Wang SW. Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis role. Biomed. Pharmacother. [Internet]. 2020; 126:110075. doi: https://doi.org/gng33r

Kolgazi M, Cilingir S, Yilmaz O, Gemici M, Yazar H, Ozer S, Acikel-Elmas M, Arbak S, Suyen GG. Caffeic acid attenuates gastric mucosal damage induced by ethanol in rats via nitric oxide modulation. Chem. Biol. Interact. [Internet]. 2021; 334:109351. doi: https://doi.org/mbk5

Qiujian Y, Zongcai F, Liping H, Jingwei H, Zhongliu Z, Fang L. Ellagic acid (EA), a tannin was isolated from Eucalyptus citriodora leaves and its anti-inflammatory activity. Med. Chem. Res. [Internet]. 2021; 30(12):2277-2288. doi: https://doi.org/mbk6

Ajiboye BO. Molecular interaction of bioactive compounds from Senecio biafrae leaf with α-amylase and α-glucosidase receptors. Clin. Phytosci. [Internet]. 2022; 8:4. doi: https://doi.org/mbk7