Inhibitory effect of the hydroalcoholic extract of "Eucalyptus" Eucalyptus globulus in bacteria isolated from mastitis bovine
Abstract
Bacteria that cause bovine mastitis can represent a risk to human health, due to high levels of antimicrobial resistance. The aim of this study was to evaluate the antimicrobial effect of Eucalyptus globulus in isolated bacteria from cows with mastitis. Eight strains of Gram-positive bacteria (4 Staphylococcus aureus and 4 coagulase negative Staphylococcus) and eight Gram negative strains (4 Klebsiella spp. and 4 Escherichia coli) were used which challenged to Eucalyptus globulus hydroalcoholic extract, determining the Minimum Inhibitory Concentration (MIC) and the inhibition diameters using the Müeller Hinton diffusion method. The CMI in Gram positive bacteria was 48.82 µg/mL, whereas in Gram negative bacteria it was 97.65 µg/mL. For a concentration of 200 mg/mL a diameter of 21.62 mm (SD = 5.01) was obtained for Gram positive bacteria and 9.6 mm (SD = 3.50) for Gram negative bacteria (p=0.0001). In 50 mg/mL of extract it was found 13.75 mm (SD = 4.70) in Gram Positives, which, in Gram Negatives, did not obtain inhibition (p˂0.0001). The results show that the hydroalcoholic extract of Eucalyptus globulus has a good antimicrobial effect against bacteria isolated from bovine mastitis, with a greater effect being observed in bacteria of the Staphylococcus genus
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