Bioactive compounds and antioxidant activity of Annona muricata L. fruits located in Manabí, Ecuador
Keywords:
soursop pulp, phenolics, antioxidant activity, acetogenins
Abstract
The mesoendocarp of Annona muricata L. (Annonaceae) has essential minerals, ascorbic acid, dietary fiber, polyphenols, and acetogenins in its composition, which evidences the nutraceutical benefits of this fruit tree. This study aimed to determine the bioactive compounds and antioxidant activity of A. muricata fruits located in Manabí, Ecuador. The content of vitamin C, dietary fiber, minerals, polyphenols, flavonoids, antioxidant activity, and the presence of acetogenins were determined. It was observed that the minerals potassium, calcium, sodium, zinc, magnesium, and iron are the major components present in soursop pulp ash. Likewise, relevant content of vitamin C (26.64 mg AA.100 g-1 pulp), total polyphenols (398.79 mg EAG.100 g-1 pulp), flavonoids (192.20 mg EQ.100 g-1 pulp), and high antioxidant activity (318.90 mmol equivalents of trolox.100 g-1 pulp) were evidenced in the fruits of A. muricata. The ethanolic extracts obtained from the fruit when applying the Kedde test, presented a pink ring, which is an unequivocal indicator of the presence of acetogenins, which have a proven antineoplasic effect. The mesoendocarp of A. muricata located in Manabí, Ecuador has an important content of bioactive compounds: essential minerals (K, Ca, Zn, Mg), vitamin C, polyphenols, presence of acetogenins and high antioxidant activity, properties that reduce the risk of degenerative diseases and cellular aging. Therefore, its consumption is recommended in the daily diet as fresh and processed fruit because it constitutes a valuable nutritional and therapeutic alternative.
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References
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AOAC. (1995). Official Methods of Analysis (16th ed). Arlington, USA: Association of Official Analytical Chemists.
Arrazola-Paternina, G. S., Barrera-Violeth, J. L. & Villalba-Cadavid, M. I. (2013). Determinación física y bromatológica de la guanábana cimarrona (Annona glabra L.) del Departamento de Córdoba. Orinoquia 17(2), 159-166. https://dialnet.unirioja.es/servlet/articulo?codigo=5615249.
Badrie, N. & Schauss, A. G. (2010). Soursop (Annona muricata L.): Composition, Nutritional Value, Medicinal Uses, and Toxicology. In: R. R. Watson and V. R. Preedy, (eds), Bioactive Foods in Promoting Health. Oxford: Academic Press, 621-643 pp. https://www.researchgate.net/publication/236834878
Baskaran, R., Ravi, R., & Rajarathnam, S. (2015). Thermal Processing Alters the Chemical Quality and Sensory Characteristics of Sweetsop (Annona squamosa L.) and Soursop (Annona muricata L.) Pulp and Nectar. Journal of Food Science 81(1), S182–S188. https://pubmed.ncbi.nlm.nih.gov/26642109/
Chan, C. L., Gan, R. Y. & Corke, H. (2016). The phenolic composition and antioxidant capacity of soluble and bound extracts in selected dietary spices and medicinal herbs. International Journal of Food Science & Technology, 51(3), 565–573. https://www.researchgate.net/publication/290437654
Clamens, C., Chacín, J., Hernández, C., Guerrero, R. & García, M. Ch. (2014). Evaluación del contenido de fenoles y vitamina C del fruto de Annona muricata L. (Guanábana) en diferentes estadios de crecimiento. Boletín del Centro de Investigaciones Biológicas 48(1), 39 – 47. https://produccioncientificaluz.org/index.php/boletin/article/view/19051
Correa, J., Ortíz, D., Larrahondo, J., Sánchez, M. & Pachón, H. (2012). Actividad antioxidante en guanábana (Annona muricata L.): una revisión bibliográfica. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 11(2), 11-126. https://www.redalyc.org/articulo.oa?id=85622734002
Coria-Téllez, A. V., Montalvo-Gónzalez, E., Yahia, E. M. & Obledo-Vázquez, E. N. (2018). Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arabian Journal of Chemistry, 11(5), 662–691. https://www.sciencedirect.com/science/article/pii/S1878535216000058.
Fernández, V., Sulbarán, B., Ojeda de Rodríguez, G., Nava, R., Delgado, J., Berradre, M. & Peña, J. (2007) Contenido mineral de la guanábana (Annona muricata) cultivada en el occidente de Venezuela. Boletín del Centro de Investigaciones Biológicas 41(1), 86-95. https://produccioncientificaluz.org/index.php/boletin/article/view/93.
Fuenmayor, M., Ettiene, G., Pérez-Pérez, E. & Raga, J. (2016). Efecto de la forma de propagación y la frecuencia de fertilización nitrogenada sobre la actividad antioxidante en frutos de guanábana (Annona muricata L.). Revista de la Facultad de Agronomía (LUZ). 33,137-161. https://produccioncientificaluz.org/index.php/agronomia/article/view/27196
García-Soto, A., Ettiene; G., Pérez-Pérez, E., Sandoval, L., Montilla, L. & Soto, E. (2012). Propagación y fertilización del cultivo del guanábano. II: Características químicas de frutos. Revista de la Facultad de Agronomía 29, 20-36. https://produccioncientificaluz.org/index.php/agronomia/article/view/26879
Gavamukulya, Y., Abou-Elella, F., Wamunyokoli, F. & AEl-Shemy, H. (2014). Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola). Asian Pacific Journal of Tropical Medicine 7, S355–S363. https://pubmed.ncbi.nlm.nih.gov/25312150/
Hernández, C., Guerrero, R., Bracho, B. & Pérez-Pérez, E. (2018). Evaluación del contenido de azúcares y perfil mineral en diferentes estadios de crecimiento del fruto de guanábana. Revista de la Facultad de Agronomía (LUZ) 35, 202-222. https://produccioncientificaluz.org/index.php/agronomia/article/view/27272
Hernández-Fuentes, G.A., García-Argáez, A.N., Peraza-Campos, A.L., Delgado-Enciso, I., Muñiz-Valencia, R., Martínez-Martínez, F.J., Toninello, A, Gómez-Sandoval, Z., Mojica-Sánchez, JP., Dalla-Via, L. & Parra-Delgado H. (2019). Cytotoxic Acetogenins from the Roots of Annona purpurea. International Journal of Molecular Sciences 20(8), 1-24. https://pubmed.ncbi.nlm.nih.gov/31014011/
Jiménez, V. M., Gruschwitz, M., Schweiggert, R. M., Carle, R., & Esquivel, P. (2014). Identification of phenolic compounds in soursop (Annona muricata) pulp by high-performance liquid chromatography with diode array and electrospray ionization mass spectrometric detection. Food Research International 65, 42–46. https://www.sciencedirect.com/science/article/abs/pii/S0963996914003664.
Jiménez-Zurita, J., Balois, R., Alia, I., Sánchez, L., Jiménez, E., Bello, J., García, J. & Juárez, P. (2017). Cold storage of two selections of Soursop (Annona muricata L.) in Nayarit, Mexico. Journal of Food Quality 1(1), 1-9. https://www.researchgate.net/publication/315057719_Cold_Storage_of_Two_Selections_of_Soursop_Annona_muricata_L_in_Nayarit_Mexico.
Laguna-Hernández, G., Brechú-Franco, A. E., De la Cruz-Chacón, I. & González-Esquinca, A. R. (2015). Histochemical detection of acetogenins and storage molecules in the endosperm of Annona macroprophyllata Donn Sm. seeds. European journal of histochemistry 59(3), 239-242. https://pubmed.ncbi.nlm.nih.gov/26428881/
Latham, M. (2002). Nutrición humana en el mundo en desarrollo. Organización de las Naciones Unidas para la Agricultura y la Alimentación Colección FAO: Alimentación y. Nutrición. N° 29. Roma, Italia. Depósito de documentos de la FAO. https://biblioteca.hegoa.ehu.eus/registros/9536
León-Méndez, G., Granados, C., Osorio, M. (2016). Caracterización de la pulpa de Annona muricata L. cultivada en el Norte del Departamento de Bolívar, Colombia. Revista Cubana de Plantas Medicinales 1(4), 175-181. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962016000400012
Liaw, C. C., Liou, J. R., Wu, T. Y., Chang, F. R. & Wu, Y-C. (2016). Acetogenins from Annonaceae. Progress in the Chemistry of Organic Natural Products 101, 113-230. https://link.springer.com/book/10.1007/978-3-319-22692-7.
McDougall, G. J. (2016). Phenolic-enriched foods: sources and processing for enhanced health benefits. Proceedings of the Nutrition Society 76(2), 163–171. https://pubmed.ncbi.nlm.nih.gov/27804893/
Nam, J., Park, Y., Jang, H. & Rhee, Y. (2017). Phenolic compounds in different parts of young Annona muricata cultivated in Korea and their antioxidant activity. Applied Biological Chemistry 60(1), 535-543. https://www.researchgate.net/publication/318843216
Oboh, G., Ademosun, A., Akinleye, M., Omojokun, O., Boligon, A. & Athayde, M. (2015). Starch composition, glycemic indices, phenolic constituents, and antioxidative and antidiabetic properties of some common tropical fruits. Journal Ethnic Foods 2(1), 264-273. https://core.ac.uk/download/pdf/82500992.pdf
Ogbu, P., Ugota, E., Onwuka, R., Ogbu, I. & Aloke, Ch. (2020) Effect of acetogenin fraction of Annona muricata leaves on antioxidant status and some indices of benign prostatic hyperplasia in rats. Redox Report 25(1), 80-86. https://pubmed.ncbi.nlm.nih.gov/32878595/
Ojeda, G., Coronado, J., Nava, R., Sulbarán, B., Araujo, D. & Cabrera, L. (2007) Caracterización fisicoquímica de la pulpa de la guanábana (Annona muricata L.) cultivada en el Occidente de Venezuela. Boletín del Centro de Investigaciones Biológicas 41(2), 151-160. https://www.researchgate.net/publication/284705114
Paul, J., Gnanam, R., Jayadeepa, R. M. & Arul, L. (2013). Anti-cancer activity on graviola, an exciting medicinal plant extract vs various cancer cell lines and a detailed computational study on its potent anti-cancerous leads. Current Topics in Medicinal Chemistry, 13(14), 1666-1673. https://pubmed.ncbi.nlm.nih.gov/23889049/.
Ramírez, R., Arenas, L., Acosta, K., Yamarte, M. & Sandoval, L. (2012). Efecto del escaldado sobre la calidad nutricional de la pulpa de guanábana (Annona muricata L.). Revista Iberoamericana de Tecnología Postcosecha 13(1), 48-57. https://www.redalyc.org/pdf/813/81324433007
Ramírez, A., & Pacheco, E. 2011. Composición química y compuestos bioactivos presentes en pulpas de piña, guayaba y guanábana. Interciencia 36(1), 71-75. https://www.redalyc.org/pdf/339/33917727011.pdf
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26, 1231-1237. https://pubmed.ncbi.nlm.nih.gov/10381194/
Sandoval, L., Ettiene, G., Pérez-Pérez, E., Fuenmayor, M., Raga, J., & Silva, N. (2014). Determinación de flavonoides en frutos de Annona muricata L. provenientes de plantas diferentes, empleando cromatografía líquida de alta resolución. Revista Facultad Agronomía-(LUZ). Supl. 1, 785-800. https://www.revfacagronluz.org.ve/PDF/suplemento_2014/ta/tasupl12014785800.pdf
Singleton V, Orthofer R., & Lamuela- Raventos, R. (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymology 299, 152-178. https://www.sciencedirect.com/science/article/abs/pii/S0076687999990171
Woisky R, & Salatino A (1998) Analysis of propolis: some parameters and procedures for chemical quality control. Journal of Apicultural Research. 37, 99-105. https://www.tandfonline.com/doi/abs/10.1080/00218839.1998.11100961
Vit, P., Santiago, B., & Pérez-Pérez, E. M. (2014). Composición química y actividad antioxidante de pulpa, hoja y semilla de guanábana Annona muricata L. Interciencia, 39(5), 350-353. https://www.redalyc.org/pdf/339/33930879008.pdf.
AOAC. (1995). Official Methods of Analysis (16th ed). Arlington, USA: Association of Official Analytical Chemists.
Arrazola-Paternina, G. S., Barrera-Violeth, J. L. & Villalba-Cadavid, M. I. (2013). Determinación física y bromatológica de la guanábana cimarrona (Annona glabra L.) del Departamento de Córdoba. Orinoquia 17(2), 159-166. https://dialnet.unirioja.es/servlet/articulo?codigo=5615249.
Badrie, N. & Schauss, A. G. (2010). Soursop (Annona muricata L.): Composition, Nutritional Value, Medicinal Uses, and Toxicology. In: R. R. Watson and V. R. Preedy, (eds), Bioactive Foods in Promoting Health. Oxford: Academic Press, 621-643 pp. https://www.researchgate.net/publication/236834878
Baskaran, R., Ravi, R., & Rajarathnam, S. (2015). Thermal Processing Alters the Chemical Quality and Sensory Characteristics of Sweetsop (Annona squamosa L.) and Soursop (Annona muricata L.) Pulp and Nectar. Journal of Food Science 81(1), S182–S188. https://pubmed.ncbi.nlm.nih.gov/26642109/
Chan, C. L., Gan, R. Y. & Corke, H. (2016). The phenolic composition and antioxidant capacity of soluble and bound extracts in selected dietary spices and medicinal herbs. International Journal of Food Science & Technology, 51(3), 565–573. https://www.researchgate.net/publication/290437654
Clamens, C., Chacín, J., Hernández, C., Guerrero, R. & García, M. Ch. (2014). Evaluación del contenido de fenoles y vitamina C del fruto de Annona muricata L. (Guanábana) en diferentes estadios de crecimiento. Boletín del Centro de Investigaciones Biológicas 48(1), 39 – 47. https://produccioncientificaluz.org/index.php/boletin/article/view/19051
Correa, J., Ortíz, D., Larrahondo, J., Sánchez, M. & Pachón, H. (2012). Actividad antioxidante en guanábana (Annona muricata L.): una revisión bibliográfica. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 11(2), 11-126. https://www.redalyc.org/articulo.oa?id=85622734002
Coria-Téllez, A. V., Montalvo-Gónzalez, E., Yahia, E. M. & Obledo-Vázquez, E. N. (2018). Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arabian Journal of Chemistry, 11(5), 662–691. https://www.sciencedirect.com/science/article/pii/S1878535216000058.
Fernández, V., Sulbarán, B., Ojeda de Rodríguez, G., Nava, R., Delgado, J., Berradre, M. & Peña, J. (2007) Contenido mineral de la guanábana (Annona muricata) cultivada en el occidente de Venezuela. Boletín del Centro de Investigaciones Biológicas 41(1), 86-95. https://produccioncientificaluz.org/index.php/boletin/article/view/93.
Fuenmayor, M., Ettiene, G., Pérez-Pérez, E. & Raga, J. (2016). Efecto de la forma de propagación y la frecuencia de fertilización nitrogenada sobre la actividad antioxidante en frutos de guanábana (Annona muricata L.). Revista de la Facultad de Agronomía (LUZ). 33,137-161. https://produccioncientificaluz.org/index.php/agronomia/article/view/27196
García-Soto, A., Ettiene; G., Pérez-Pérez, E., Sandoval, L., Montilla, L. & Soto, E. (2012). Propagación y fertilización del cultivo del guanábano. II: Características químicas de frutos. Revista de la Facultad de Agronomía 29, 20-36. https://produccioncientificaluz.org/index.php/agronomia/article/view/26879
Gavamukulya, Y., Abou-Elella, F., Wamunyokoli, F. & AEl-Shemy, H. (2014). Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola). Asian Pacific Journal of Tropical Medicine 7, S355–S363. https://pubmed.ncbi.nlm.nih.gov/25312150/
Hernández, C., Guerrero, R., Bracho, B. & Pérez-Pérez, E. (2018). Evaluación del contenido de azúcares y perfil mineral en diferentes estadios de crecimiento del fruto de guanábana. Revista de la Facultad de Agronomía (LUZ) 35, 202-222. https://produccioncientificaluz.org/index.php/agronomia/article/view/27272
Hernández-Fuentes, G.A., García-Argáez, A.N., Peraza-Campos, A.L., Delgado-Enciso, I., Muñiz-Valencia, R., Martínez-Martínez, F.J., Toninello, A, Gómez-Sandoval, Z., Mojica-Sánchez, JP., Dalla-Via, L. & Parra-Delgado H. (2019). Cytotoxic Acetogenins from the Roots of Annona purpurea. International Journal of Molecular Sciences 20(8), 1-24. https://pubmed.ncbi.nlm.nih.gov/31014011/
Jiménez, V. M., Gruschwitz, M., Schweiggert, R. M., Carle, R., & Esquivel, P. (2014). Identification of phenolic compounds in soursop (Annona muricata) pulp by high-performance liquid chromatography with diode array and electrospray ionization mass spectrometric detection. Food Research International 65, 42–46. https://www.sciencedirect.com/science/article/abs/pii/S0963996914003664.
Jiménez-Zurita, J., Balois, R., Alia, I., Sánchez, L., Jiménez, E., Bello, J., García, J. & Juárez, P. (2017). Cold storage of two selections of Soursop (Annona muricata L.) in Nayarit, Mexico. Journal of Food Quality 1(1), 1-9. https://www.researchgate.net/publication/315057719_Cold_Storage_of_Two_Selections_of_Soursop_Annona_muricata_L_in_Nayarit_Mexico.
Laguna-Hernández, G., Brechú-Franco, A. E., De la Cruz-Chacón, I. & González-Esquinca, A. R. (2015). Histochemical detection of acetogenins and storage molecules in the endosperm of Annona macroprophyllata Donn Sm. seeds. European journal of histochemistry 59(3), 239-242. https://pubmed.ncbi.nlm.nih.gov/26428881/
Latham, M. (2002). Nutrición humana en el mundo en desarrollo. Organización de las Naciones Unidas para la Agricultura y la Alimentación Colección FAO: Alimentación y. Nutrición. N° 29. Roma, Italia. Depósito de documentos de la FAO. https://biblioteca.hegoa.ehu.eus/registros/9536
León-Méndez, G., Granados, C., Osorio, M. (2016). Caracterización de la pulpa de Annona muricata L. cultivada en el Norte del Departamento de Bolívar, Colombia. Revista Cubana de Plantas Medicinales 1(4), 175-181. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962016000400012
Liaw, C. C., Liou, J. R., Wu, T. Y., Chang, F. R. & Wu, Y-C. (2016). Acetogenins from Annonaceae. Progress in the Chemistry of Organic Natural Products 101, 113-230. https://link.springer.com/book/10.1007/978-3-319-22692-7.
McDougall, G. J. (2016). Phenolic-enriched foods: sources and processing for enhanced health benefits. Proceedings of the Nutrition Society 76(2), 163–171. https://pubmed.ncbi.nlm.nih.gov/27804893/
Nam, J., Park, Y., Jang, H. & Rhee, Y. (2017). Phenolic compounds in different parts of young Annona muricata cultivated in Korea and their antioxidant activity. Applied Biological Chemistry 60(1), 535-543. https://www.researchgate.net/publication/318843216
Oboh, G., Ademosun, A., Akinleye, M., Omojokun, O., Boligon, A. & Athayde, M. (2015). Starch composition, glycemic indices, phenolic constituents, and antioxidative and antidiabetic properties of some common tropical fruits. Journal Ethnic Foods 2(1), 264-273. https://core.ac.uk/download/pdf/82500992.pdf
Ogbu, P., Ugota, E., Onwuka, R., Ogbu, I. & Aloke, Ch. (2020) Effect of acetogenin fraction of Annona muricata leaves on antioxidant status and some indices of benign prostatic hyperplasia in rats. Redox Report 25(1), 80-86. https://pubmed.ncbi.nlm.nih.gov/32878595/
Ojeda, G., Coronado, J., Nava, R., Sulbarán, B., Araujo, D. & Cabrera, L. (2007) Caracterización fisicoquímica de la pulpa de la guanábana (Annona muricata L.) cultivada en el Occidente de Venezuela. Boletín del Centro de Investigaciones Biológicas 41(2), 151-160. https://www.researchgate.net/publication/284705114
Paul, J., Gnanam, R., Jayadeepa, R. M. & Arul, L. (2013). Anti-cancer activity on graviola, an exciting medicinal plant extract vs various cancer cell lines and a detailed computational study on its potent anti-cancerous leads. Current Topics in Medicinal Chemistry, 13(14), 1666-1673. https://pubmed.ncbi.nlm.nih.gov/23889049/.
Ramírez, R., Arenas, L., Acosta, K., Yamarte, M. & Sandoval, L. (2012). Efecto del escaldado sobre la calidad nutricional de la pulpa de guanábana (Annona muricata L.). Revista Iberoamericana de Tecnología Postcosecha 13(1), 48-57. https://www.redalyc.org/pdf/813/81324433007
Ramírez, A., & Pacheco, E. 2011. Composición química y compuestos bioactivos presentes en pulpas de piña, guayaba y guanábana. Interciencia 36(1), 71-75. https://www.redalyc.org/pdf/339/33917727011.pdf
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26, 1231-1237. https://pubmed.ncbi.nlm.nih.gov/10381194/
Sandoval, L., Ettiene, G., Pérez-Pérez, E., Fuenmayor, M., Raga, J., & Silva, N. (2014). Determinación de flavonoides en frutos de Annona muricata L. provenientes de plantas diferentes, empleando cromatografía líquida de alta resolución. Revista Facultad Agronomía-(LUZ). Supl. 1, 785-800. https://www.revfacagronluz.org.ve/PDF/suplemento_2014/ta/tasupl12014785800.pdf
Singleton V, Orthofer R., & Lamuela- Raventos, R. (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymology 299, 152-178. https://www.sciencedirect.com/science/article/abs/pii/S0076687999990171
Woisky R, & Salatino A (1998) Analysis of propolis: some parameters and procedures for chemical quality control. Journal of Apicultural Research. 37, 99-105. https://www.tandfonline.com/doi/abs/10.1080/00218839.1998.11100961
Vit, P., Santiago, B., & Pérez-Pérez, E. M. (2014). Composición química y actividad antioxidante de pulpa, hoja y semilla de guanábana Annona muricata L. Interciencia, 39(5), 350-353. https://www.redalyc.org/pdf/339/33930879008.pdf.
Published
2024-02-27
How to Cite
Párraga, C., Rincón-Acosta, F., Barre-Zambrano, R., Vera-Cedeño, J., Vargas, P., & Mendoza , F. (2024). Bioactive compounds and antioxidant activity of Annona muricata L. fruits located in Manabí, Ecuador. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 41(1), e244107. Retrieved from https://mail.produccioncientificaluz.org/index.php/agronomia/article/view/41697
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Food Technology
Copyright (c) 2024 Cecilia Párraga, Fernando Rincón-Acosta, Roy Barre-Zambrano, Jhon Vera-Cedeño, Plinio Vargas, Freddy Mendoza Rivadeneira
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
