Análise de Glyoxalase I (GLX-I) em milho nativo de Oaxaca, México, infectado com Aspergillus flavus in vitro
Palavras-chave:
GLX-I, estresse, proteínas, Aspergillus
Resumo
O sistema glioxalase desempenha um papel importante em vários processos fisiológicos em plantas quando estas são submetidas a diferentes tipos de estresse, seja físico, químico ou biológico. Aspergillus flavus é um fungo produtor de aflatoxinas que contamina grãos secos, levando a uma deterioração gradual dos grãos e uma redução significativa em seu valor nutricional. O objetivo do presente estudo foi avaliar a atividade da enzima glioxalase I (GLX-I) em coleóptilos de milho de Oaxaca em resposta à infecção causada por Aspergillus flavus. Nove amostras de milho de quatro raças diferentes foram analisadas. As amostras foram inoculadas com uma suspensão de esporos de Aspergillus flavus de concentração conhecida e a extração e quantificação da proteína total foram realizadas nos coleóptilos, e a atividade do GLX-I foi determinada pela quantificação da quantidade de S-lactoilglutationa produzida por minuto. Além disso, foi realizada a análise da expressão gênica por reação em cadeia da polimerase com transcriptase reversa (RT-PCR). Os coleóptilos de milho inoculados apresentaram sintomas de infecção, alterações de cor e murchamento. A concentração de proteínas totais diminuiu significativamente nos extratos de quatro amostras na presença do fungo. Na análise de GLX-I, duas amostras apresentaram a maior atividade enzimática no extrato de coleóptilo infectado em relação ao saudável, além de apresentarem maior expressão do gene no ensaio de RT-PCR, isso devido à resposta à infecção por Aspergillus flavus.
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Referências
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Bandyopadhyay R., Ortega-Beltrán A., Akande A., Mutegi C., Atehnkeng J., Kaptoge L., Senghor A. L., Adhikari B. N. & Cotty P. J. (2016). Biological control of aflatoxins in Africa: current status and potential challenges in the face of climate change. World Mycitoxin Journal, 9(5): 771-789. https://doi.org/10.3920/WMJ2016.2130
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Ben M. R., El-Omari R., El-Mourabit N., Bouargalne Y. & NHIRI M. (2018). Changes in the antioxidant and glyoxalase enzyme activities in leaves of two Moroccan sorghum ecotypes with differential tolerance to nitrogen stress. Australian Journal of Crop Science, 12(8): 1280-1287. https://www.cropj.com/mrid_12_8_2018_1280_1287.pdf
Borysiuk K., Ostaszewska-Bugajska M., Vaultier M-N, Hasenfratz-Sauder M-P & Szal B. (2018). Enhanced formation of methylglyoxal-derived advanced glycation end products in Arabidopsis under Ammonium Nutrition. Frontiers in Plant Science, 9: 667. https://doi.org/10.3389/fpls.2018.00667
Cabrera T. J. M., Carballo A. & Aragón-Cuevas F. (2015). Evaluación agronómica de maíces raza Zapalote chico en la región Istmeña de Oaxaca. Revista Mexicana Ciencias Agrícolas, 11: 2075-2082. https://doi.org/10.29312/remexca.v0i11.775
Chen Z. Y., Brown R. L., Damann K. E. & Cleveland T. E. (2004). Identification of a maize kernel stress-related protein and its effect on aflatoxin accumulation. Phytopathology, 94(9): 938-945. https://doi.org/10.1094/PHYTO.2004.94.9.938
Chen, Z.-Y., Brown R. L., Damann K. E. & Cleveland T. E. (2007). Identification of maize kernel endosperm proteins associated with resistance to aflatoxin contamination by Aspergillus flavus. Phytopathology, 97(9): 1094–1103. https://doi.org/10.1094/PHYTO-97-9-1094
Chomczynski P. and Sacchi N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, 162(1):156-9. https://doi.org/10.1016/0003-2697(87)90021-2
Fernández S. R., Morales L. A. & A. Gálvez M. (2013). Importancia de los maíces nativos de México en la dieta nacional. Una revisión indispensable. Revista Fitotecnia Mexicana, 36(3-A): 275-283. http://www.scielo.org.mx/pdf/rfm/v36s3-a/v36s3-aa4.pdf
Fountain J. C., Chen Z-Y, Scully B. T., Kamerait R. C., Lee R. D. & Guo B. (2010). Pathogenesis-related gene expressions in different maize genotypes under drought stressed conditions. African Journal Plant Science, 4(11): 433-440. https://academicjournals.org/article/article1380126077_Fountain%20et%20al.pdf
Ghosh A. (2017). Genome-wide identification of glyoxalase genes in Medicago truncatula and their expression profiling in response to various developmental and environmental stimuli. Frontiers in Plant Science, 8: 836. https://doi.org/10.3389/fpls.2017.00836
Ghosh A., Kushwana H. R., Hasan M. R., Pareek A., Sopory S. K. & Singla-Pareek S. L. (2016). Presence of unique glyoxalase III proteins in plants indicates the existence of shorter route for methylglyoxal detoxification. Scientific Reports, 6:18358. https://doi.org/10.1038/srep18358
Hasanuzzaman M., Nahar K., Hossain Md. S., Mahmud J. A., Rahman A., Inafuku M., Oku H. & Fujita M. (2017a). Coordinated actions of glyoxalase and antioxidant defense systems in conferring abiotic stress tolerance in plants. International Journal Molecular Sciences, 18(1): 200. https://doi.org/10.3390/ijms18010200
Hasanuzzaman M., Nahar K., Hossain Md. S., Anee T. I., Parvin K. & Fujita M. (2017b). Nitric oxide pretreatment enhances antioxidant defense and glyoxalase systems to confer PEG-induced oxidative stress in rapeseed. Journal of Plant Interaction, 12(1): 323-331. https://doi.org/10.1080/17429145.2017.1362052
Kato Y. T. A., Mapes C., Mera L. M., Serratos J. A. & Bye R. A. (2009). Origen y diversificación del maíz; Una revisión analítica. UNAM: México: 115 pp. https://issuu.com/perrasdelmal/docs/origen_y_diversif._del_maiz
Kaur C., Sharma S., Hasan M. R., Pareek A., Singla-Pareek S. L. & Sopory S. K. (2017). Characteristic variations and similarities in biochemical, molecular and functional Properties of glyoxalases across Prokaryotes and Eukaryotes. International Journal of Molecular Sciences, 18:250. https://doi.org/10.3390/ijms18040250
Li Z-G. (2016). Methylglyoxal and glyoxalase system in plants: old players, new concepts. The Botanical Review, 82: 183-203. https://doi.org/10.1007/s12229-016-9167-9
Rosado O. A. and Villasante S. B. (2021). Los herederos del maíz. Instituto Nacional de los Pueblos Indígenas, Ciudad de México. https://www.gob.mx/cms/uploads/attachment/file/658352/Libro-Los-herederos-del-maiz-INPI.pdf
Martínez P. H. Y., Hernández S., Reyes C. A. & Vázquez-Carrillo G. (2013). El género Aspergillus y sus micotoxinas de maíz en México: problemática y perspectivas. Revista Mexicana de Fitopatología, 31(2): 126-146. http://www.scielo.org.mx/pdf/rmfi/v31n2/v31n2a5.pdf
Orozco-Ramírez Q., Ross-Ibarra J., Santacruz-Varela A. & Brush S. (2016). Maize diversity associated with social origin and environmental variation in Southern Mexico. Heredity, 116(5): 477-484. https://doi.org/10.1038/hdy.2016.10
Rajasekaran K., Sayler R. J., Majumdar R., Sickler C. M. & Cary J. W. (2019). Inhibition of Aspergillus flavus growth and aflatoxin production in transgenic maize expressing the α-amylase inhibitor from Lablab purpureus L. Journal of Visualized Experiments, 144. https://doi.org/10.3791/59169
Rohman Md. M., Talukder M. Z. A., Hossain M. G., Uddin M. S., Amiruzzaman M., Biswas A., Ahsan A. F. M. & Chowdhury M. A. Z. (2016). Saline sensitivity leads to oxidative stress and increases the antioxidants in presence of proline and betaine in maize (Zea mays L.) inbred. Plants Omics Journal, 9(1): 35-47. omics.com/rohman_9_1_2016-35_47.pdf
Sadiq I., Fanucchi F., Paparelli E., Alpi E., Bachi A., Alpi A. & Perata P. (2011). Proteomic identification of differentially expressed proteins in the anoxic rice coleoptile. Journal of Plant Physiology, 168:2234-2243. https://doi.org/10.1016/j.jplph.2011.07.009
Servicio de Información Agroalimentaria y Pesquera. SIAP. (2021). Anuario estadístico de la producción agrícola. Consultado el 9 de mayo de 2022. https://nube.siap.gob.mx/cierreagricola/
Schneider C., Rasband W. & Eliceiri, K. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9 (7): 671–675. https://doi.org/10.1038/nmeth.2089
Turra G. L., Agostini R. B., Fauguel C. M., Presello D. A., Andreo C. S., González J. M. & Campos-Bermudez V. A. (2015). Structure of the novel monomeric glyoxalase I from Zea mays. Acta Crystallogr D Biol Crystallogr, 71(10): 2009-2020.
https://doi.org/10.1107/S1399004715015205
Zhou Z-H, Wang Y., Ye X-Y & Li Z-G. (2018). Signaling molecule hydrogen sulfide improves seed germination and seedling growth of Maize (Zea mays L.) under high temperature by inducing antioxidant system and osmolyte biosynthesis. Frontiers in Plant Science, 9: 1288. https://doi.org/10.3389/fpls.2018.01288
Varapizuela S. C. F., Sánchez M. M. A. & Pérez S. A. D. (2019). Análisis de la actividad enzimática de peroxidasas en maíces nativos de Oaxaca infectados por Aspergillus parasiticus. Revista Ingeniantes, 2(2): 75-79. https://citt.itsm.edu.mx/ingeniantes/pdfversion/ingeniantes6no2vol2-esp.pdf
Aragón C. F., Taba S, Hernández J. M., Figueroa J. D. & Serrano V. (2006). Actualización de la información sobre los maíces criollos de Oaxaca. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Informe final SNIB-CONABIO proyecto No. CS002 México D. F. http://www.conabio.gob.mx/institucion/proyectos/resultados/InfCS002.pdf
Arteaga M. C., Moreno-Letelier A., Mastretta-Yanes A., Vázquez-Lobo A., Breña-Ochoa A., Moreno-Estrada A., Eguiarte L. E. & Piñero D. (2016). Genomic variation in recently collected maize landraces from Mexico. Genomics Data, 7: 38-45. https://doi.org/10.1016/j.gdata.2015.11.002
Bandyopadhyay R., Ortega-Beltrán A., Akande A., Mutegi C., Atehnkeng J., Kaptoge L., Senghor A. L., Adhikari B. N. & Cotty P. J. (2016). Biological control of aflatoxins in Africa: current status and potential challenges in the face of climate change. World Mycitoxin Journal, 9(5): 771-789. https://doi.org/10.3920/WMJ2016.2130
Bania I. and Mahanta R. (2012). Evaluation of peroxidases from various plant sources. International Journal of Scientific and Research Publications, 2(5). http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.387.3615&rep=rep1&type=pdf
Baker R. L., Brown R. L., Chen Z. Y., Cleveland T. E. & Fakhoury A. M. (2009). A maize lectin-like protein with antifungal activity against Aspergillus flavus. Journal of food protection, 72(1): 120-127. https://doi.org/10.4315/0362-028X-72.1.120
Ben M. R., El-Omari R., El-Mourabit N., Bouargalne Y. & NHIRI M. (2018). Changes in the antioxidant and glyoxalase enzyme activities in leaves of two Moroccan sorghum ecotypes with differential tolerance to nitrogen stress. Australian Journal of Crop Science, 12(8): 1280-1287. https://www.cropj.com/mrid_12_8_2018_1280_1287.pdf
Borysiuk K., Ostaszewska-Bugajska M., Vaultier M-N, Hasenfratz-Sauder M-P & Szal B. (2018). Enhanced formation of methylglyoxal-derived advanced glycation end products in Arabidopsis under Ammonium Nutrition. Frontiers in Plant Science, 9: 667. https://doi.org/10.3389/fpls.2018.00667
Cabrera T. J. M., Carballo A. & Aragón-Cuevas F. (2015). Evaluación agronómica de maíces raza Zapalote chico en la región Istmeña de Oaxaca. Revista Mexicana Ciencias Agrícolas, 11: 2075-2082. https://doi.org/10.29312/remexca.v0i11.775
Chen Z. Y., Brown R. L., Damann K. E. & Cleveland T. E. (2004). Identification of a maize kernel stress-related protein and its effect on aflatoxin accumulation. Phytopathology, 94(9): 938-945. https://doi.org/10.1094/PHYTO.2004.94.9.938
Chen, Z.-Y., Brown R. L., Damann K. E. & Cleveland T. E. (2007). Identification of maize kernel endosperm proteins associated with resistance to aflatoxin contamination by Aspergillus flavus. Phytopathology, 97(9): 1094–1103. https://doi.org/10.1094/PHYTO-97-9-1094
Chomczynski P. and Sacchi N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, 162(1):156-9. https://doi.org/10.1016/0003-2697(87)90021-2
Fernández S. R., Morales L. A. & A. Gálvez M. (2013). Importancia de los maíces nativos de México en la dieta nacional. Una revisión indispensable. Revista Fitotecnia Mexicana, 36(3-A): 275-283. http://www.scielo.org.mx/pdf/rfm/v36s3-a/v36s3-aa4.pdf
Fountain J. C., Chen Z-Y, Scully B. T., Kamerait R. C., Lee R. D. & Guo B. (2010). Pathogenesis-related gene expressions in different maize genotypes under drought stressed conditions. African Journal Plant Science, 4(11): 433-440. https://academicjournals.org/article/article1380126077_Fountain%20et%20al.pdf
Ghosh A. (2017). Genome-wide identification of glyoxalase genes in Medicago truncatula and their expression profiling in response to various developmental and environmental stimuli. Frontiers in Plant Science, 8: 836. https://doi.org/10.3389/fpls.2017.00836
Ghosh A., Kushwana H. R., Hasan M. R., Pareek A., Sopory S. K. & Singla-Pareek S. L. (2016). Presence of unique glyoxalase III proteins in plants indicates the existence of shorter route for methylglyoxal detoxification. Scientific Reports, 6:18358. https://doi.org/10.1038/srep18358
Hasanuzzaman M., Nahar K., Hossain Md. S., Mahmud J. A., Rahman A., Inafuku M., Oku H. & Fujita M. (2017a). Coordinated actions of glyoxalase and antioxidant defense systems in conferring abiotic stress tolerance in plants. International Journal Molecular Sciences, 18(1): 200. https://doi.org/10.3390/ijms18010200
Hasanuzzaman M., Nahar K., Hossain Md. S., Anee T. I., Parvin K. & Fujita M. (2017b). Nitric oxide pretreatment enhances antioxidant defense and glyoxalase systems to confer PEG-induced oxidative stress in rapeseed. Journal of Plant Interaction, 12(1): 323-331. https://doi.org/10.1080/17429145.2017.1362052
Kato Y. T. A., Mapes C., Mera L. M., Serratos J. A. & Bye R. A. (2009). Origen y diversificación del maíz; Una revisión analítica. UNAM: México: 115 pp. https://issuu.com/perrasdelmal/docs/origen_y_diversif._del_maiz
Kaur C., Sharma S., Hasan M. R., Pareek A., Singla-Pareek S. L. & Sopory S. K. (2017). Characteristic variations and similarities in biochemical, molecular and functional Properties of glyoxalases across Prokaryotes and Eukaryotes. International Journal of Molecular Sciences, 18:250. https://doi.org/10.3390/ijms18040250
Li Z-G. (2016). Methylglyoxal and glyoxalase system in plants: old players, new concepts. The Botanical Review, 82: 183-203. https://doi.org/10.1007/s12229-016-9167-9
Rosado O. A. and Villasante S. B. (2021). Los herederos del maíz. Instituto Nacional de los Pueblos Indígenas, Ciudad de México. https://www.gob.mx/cms/uploads/attachment/file/658352/Libro-Los-herederos-del-maiz-INPI.pdf
Martínez P. H. Y., Hernández S., Reyes C. A. & Vázquez-Carrillo G. (2013). El género Aspergillus y sus micotoxinas de maíz en México: problemática y perspectivas. Revista Mexicana de Fitopatología, 31(2): 126-146. http://www.scielo.org.mx/pdf/rmfi/v31n2/v31n2a5.pdf
Orozco-Ramírez Q., Ross-Ibarra J., Santacruz-Varela A. & Brush S. (2016). Maize diversity associated with social origin and environmental variation in Southern Mexico. Heredity, 116(5): 477-484. https://doi.org/10.1038/hdy.2016.10
Rajasekaran K., Sayler R. J., Majumdar R., Sickler C. M. & Cary J. W. (2019). Inhibition of Aspergillus flavus growth and aflatoxin production in transgenic maize expressing the α-amylase inhibitor from Lablab purpureus L. Journal of Visualized Experiments, 144. https://doi.org/10.3791/59169
Rohman Md. M., Talukder M. Z. A., Hossain M. G., Uddin M. S., Amiruzzaman M., Biswas A., Ahsan A. F. M. & Chowdhury M. A. Z. (2016). Saline sensitivity leads to oxidative stress and increases the antioxidants in presence of proline and betaine in maize (Zea mays L.) inbred. Plants Omics Journal, 9(1): 35-47. omics.com/rohman_9_1_2016-35_47.pdf
Sadiq I., Fanucchi F., Paparelli E., Alpi E., Bachi A., Alpi A. & Perata P. (2011). Proteomic identification of differentially expressed proteins in the anoxic rice coleoptile. Journal of Plant Physiology, 168:2234-2243. https://doi.org/10.1016/j.jplph.2011.07.009
Servicio de Información Agroalimentaria y Pesquera. SIAP. (2021). Anuario estadístico de la producción agrícola. Consultado el 9 de mayo de 2022. https://nube.siap.gob.mx/cierreagricola/
Schneider C., Rasband W. & Eliceiri, K. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9 (7): 671–675. https://doi.org/10.1038/nmeth.2089
Turra G. L., Agostini R. B., Fauguel C. M., Presello D. A., Andreo C. S., González J. M. & Campos-Bermudez V. A. (2015). Structure of the novel monomeric glyoxalase I from Zea mays. Acta Crystallogr D Biol Crystallogr, 71(10): 2009-2020.
https://doi.org/10.1107/S1399004715015205
Zhou Z-H, Wang Y., Ye X-Y & Li Z-G. (2018). Signaling molecule hydrogen sulfide improves seed germination and seedling growth of Maize (Zea mays L.) under high temperature by inducing antioxidant system and osmolyte biosynthesis. Frontiers in Plant Science, 9: 1288. https://doi.org/10.3389/fpls.2018.01288
Varapizuela S. C. F., Sánchez M. M. A. & Pérez S. A. D. (2019). Análisis de la actividad enzimática de peroxidasas en maíces nativos de Oaxaca infectados por Aspergillus parasiticus. Revista Ingeniantes, 2(2): 75-79. https://citt.itsm.edu.mx/ingeniantes/pdfversion/ingeniantes6no2vol2-esp.pdf
Publicado
2022-09-21
Como Citar
Varapizuela-Sánchez, C. F., Sánchez-Medina, M. A., Pina-Canseco, M. del S., Rosas-Murrieta, N. H., Pérez-Santiago, A. D., & García-Montalvo, I. A. (2022). Análise de Glyoxalase I (GLX-I) em milho nativo de Oaxaca, México, infectado com Aspergillus flavus in vitro. Revista Da Faculdade De Agronomia Da Universidade De Zulia, 39(4), e223946. Obtido de https://mail.produccioncientificaluz.org/index.php/agronomia/article/view/38769
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Direitos de Autor (c) 2022 Carlos Francisco Varapizuela-Sánchez, Marco Antonio Sánchez-Medina, María del Socorro Pina-Canseco, Nora Hilda Rosas-Murrieta, Alma Dolores Pérez-Santiago, Iván Antonio García-Montalvo
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