Inmunodiagnóstico de la toxoplasmosis: Hemaglutinación indirecta e inmunoanálisis enzimático IgM e IgG
Abstract
En el presente trabajo se compararon los métodos de hemaglutinación Indirecta (H.A.l.) e Inmunoanálisis Enzimático (E.L.l.S.A IgM e IgG) para investigar anticuerpos antitoxoplasma en 54 muestras sanguíneas. Los resultados obtenidos muestran una concordancia del 62.96% entre H.A.I. y E.L.I.S.A IgM y del 83.33% entre H.A.I. y E.L.I.S.A IgG. El análisis estadístico mediante el Chi cuadrado, reveló que hubo significancia cuando se compararon todos los títulos de H.A.I. a partir de 1:2 con todos los índices de E.L.I.S.A IgM, esta significancia no puede establecer la superioridad de un método sobre el otro, debido: a. La utilización del ";kit"; comercial E.L.I.S.A. IgM que sólo detecta este tipo de anticuerpo; b. La detección por H.A.I. de IgM e IgG; c. La presencia de anticuerpos inespecíficos por H.A.I. Por otra parte, el Chi cuadrado para los métodos de H.A.I. y E.L.I.S.A. IgG reveló que no hubo diferencias, lo cual nos indica que la discordancia entre ambos métodos no es estadísticamente significante.
Copyright (c) 2012 T. Romero, E. Pozo, M. Ruiz, A. Montiel
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