Effect of the essential oil of Lippia graveolens in the biological control of Fusarium chlamydosporum

• Jesús Di Carlo Quiroz Velásquez Instituto Politécnico Nacional. Reynosa, Tamaulipas, México. https://orcid.org/0000-0002-6021-0427
• Guadalupe C. Rodríguez Castillejos Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México. https://orcid.org/0000-0003-0205-9340
• Israel García León Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México. https://orcid.org/0000-0002-7876-1219
• Cristian Lizarazo Ortega Instituto Politécnico Nacional. Reynosa, Tamaulipas, México. https://orcid.org/0000-0002-0010-9386
• Jesús G. García Olivares Instituto Politécnico Nacional. Reynosa, Tamaulipas, México. https://orcid.org/0000-0002-3826-2968
• J. Luis Hernández Mendoza Instituto Politécnico Nacional. Reynosa, Tamaulipas, México. https://orcid.org/0000-0002-1233-0133

DOI: https://doi.org/10.46925//rdluz.37.02

Keywords: Antagonism, ethanolic extracts, antibiosis, bioassays, in vitro assays

Abstract

Essential oils are characterized by having smell, flavor and often antifungal properties;They are produced by aromatic and medicinal plants. It is important to determine what effect the essential oils of L graveolens have in the control of phytopathogens. The evaluation of the essential oil of Lippia graveolens was carried out on the fungus Fusarium chlamydosporum, which has a high incidence, reduces productivity and can affect humans. The extraction of the oils was carried out in absolute ethanol. DNA extraction was performed from the biomass and a PCR was performed using the oligonucleotides ITS1 and ITS4. The fungus was confronted in petri dishes with the essential oils of L graveolens. Lower growth of Fusarium sp was observed in the Viad 01 sample, with 1.23 cm of growth; while in the control a colony of 4.60 cm was obtained, which represents a difference of 3.37 cm with respect to the Viad 01 sample. This shows that Lippia graveolens extracts can be an alternative for disease control by innovating for the sustainable and competitive agriculture, in addition to its potential use in human health..

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