Evaluation of chlorophyll content and membrane stability under oxidative stress induced by glyphosate herbicide as indicators of drought tolerance in some advanced durum wheat (Triticum durum L.) lines: in vitro study
Keywords:
electrolyte leakage, chlorophyll loss, tolerant, sensitivity
Abstract
Oxidative stress caused by glyphosate is a complex chemical and physiological phenomenon and develops as a result of overproduction and accumulation of reactive oxygen species (ROS). This study was carried out in vitro at the National Institute of Agronomic Research of Algeria (INRAA) Setif, to select the most susceptible durum wheat (Triticum durum L.) under oxidative stress induced by glyphosate herbicide by evaluating chlorophyll content degradation and cell membrane leakage. Genotypes showed significant variations in almost all the studied traits. The chlorophyll loss ratio ranged from 26.42 % for the genotype G5 to 48.75 % for the local variety Boutaleb, glyphosate sensitivity index values were found to be between 0.65-1.2, the advanced line G5 was found to be the most tolerant under oxidative stress with the lowest chlorophyll loss ratio and lowest Glyphosate sensitivity index. Furthermore, the advanced line G4 recorded the highest electrolyte leakage (80.16 %) while G6 showed the lowest estimate (50.77 %). Therefore, advanced lines G5 and G6 appear the most suitable for the growing conditions.
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References
Ahsan, N., Lee, D.G., Lee, K.W., Alam, I., Lee, S.H., Bahk, J.D., Lee, B.H. (2008). Glyphosate induced oxidative stress in rice leaves revealed by proteomic approach. Plant Physiology and Biochemistry 46, 1062–1070. https://doi.org/10.1016/j.plaphy.2008.07.002.
Bajji, M., Lutts, S and Kinet, J.M. (2001). Water deficit effects on solute contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions. Plant Science 160(4): 669–681. https://doi.org/10.1016/S0168-9452(00)00443-X.
Bali, A.S., Sidhu, G.P.S. (2019). Abiotic Stress-Induced Oxidative Stress in Wheat. M. Hasanuzzaman et al. (eds.), Wheat Production in Changing Environments, Springer Nature Singapore Pte Ltd. https://doi.org/10.1079/9781789248098.0019.
Caglar, O., Ozturk, A., Aydin, M., Bayram, S. (2011). Paraquat tolerance of bread wheat (Triticum aestivum L.) Genotypes. Journal of animal and veterinary advances 10: 3363-3367. https://doi.org/10.3923/javaa.2011.3363.3367.
Enneb, H., Ben Yahya, L., Ilyas, M., Asaram Dhale, D., Bagues, M and Nagaz, K. (2020). Influence of Water Stress on Growth, Chlorophyll Contents and Solute Accumulation in Three Accessions of Vicia faba L.from Tunisian Arid Region. Abiotic stress in plants. http://dx.doi.org/10.5772/intechopen.94563.
FAO. Food and Agriculture Organization. Crop Prospects and Food Situation - Quarterly Global Report n°. 4, December (2020). Rome. http://www.fao.org/faostat/en/#data/QC.
Freitas-Silva, L., Rodríguez-Ruiz, M., Houmani, H., da Silva, L.C., Palma, J.M., and Corpas F.J. (2017). Glyphosate-induced oxidative stress in Arabidopsis thaliana affecting peroxisomal metabolism and triggers activity in the oxidative phase of the pentose phosphate pathway (OxPPP) involved in NADPH generation. Journal Plant Physiology. 218: 196–205. https://doi.org/10.1016/j.jplph.2017.08.007.
Gomes, M. P., Le Manac’h, S. G., Hénault-Ethier, L., Labrecque, M., Lucotte, M and Juneau, P. (2017). Glyphosate-Dependent Inhibition of Photosynthesis in Willow. Frontiers in Plant Science. 8: 207. https://doi.org/10.3389/fpls.2017.00207.
Gomes, M.P., Smedbol, E., Chalifour, A, Hénault-Ethier, L., Labrecque, M., Lepage, L., Lucotte, M., Juneau, P. (2014). Alteration of plant physiology by glyphosate and its by-product aminomethylphosphonic acid: an overview. Journal of Experimental Botany, Vol. 65, No. 17, pp. 4691–4703. https://doi.org/10.1093/jxb/eru269.
Ibrahim, R.I.H., Alkhudairi, U.A., Alhusayni, S.A.S. (2022). Alleviation of Herbicide Toxicity in Solanum lycopersicum L.-An Antioxidant Stimulation Approach. Plants 2022, 11(17), 2261. https://doi.org/10.3390/plants11172261.
Karabulut, F., and Çanakcı, S. (2021). Effects of Glyphosate Herbicide on Photosynthetic Pigments and Antioxidant Enzyme Activities in Corn (Zea mays L.) and Wheat (Triticum aestivum L.) Varieties. Journal of Physical Chemistry and Functional Materials. Volume 4, Issue 2 (2021) 61-66. https://doi.org/10.54565/jphcfum.1004433.
Khalilzadeh, R., Sharifi, R., and Jalilian, J. (2016). Antioxidant status and physiological responses of wheat (Triticum aestivum L.) to cycocel application and bio fertilizers under water limitation condition. Journal of Plant Interactions, 11:1, 130-137. https://doi.org/10.1080/17429145.2016.1221150.
Malalgoda, M., Ohm, J.B, Howatt, K.A., Simsek, S. (2020). Pre-harvest glyphosate application and effects on wheat starch chemistry: Analysis from application to harvest. Journal of Food Biochemistry. https://doi.org/10.1111/jfbc.13330.
Masoumi, A., Kafi, M., Khazaei, H., Davari, K. (2010). Effect of drought stress on water status, electrolyte leakage and enzymatic antioxidants of kochia (kochia scoparia) under saline condition. Pakistan Journal of Botany 42(5): 3517-3524.
Oulmi, A., and Aissaoui, M.R. (2022). Canopy temperature and chlorophyll content as plant traits indicators for durum wheat (Triticum durum Desf. ) superior lines selection under semi-arid conditions. Agricultural Science and Technology, VOL. 14, (2), 114-122. https://doi.org/10.15547/ast.2022.02.027.
Rivas-Garcia, T., Espinosa-Calderón, A., Hernández-Vázquez, B., and Schwentesius-Rindermann, R. (2022). Overview of Environmental and Health Effects Related to Glyphosate Usage. Sustainability, 14(11), 6868. https://doi.org/10.3390/su14116868.
Sakya, A.T., Sulistyaningsih, E., Indradewa, D., and Purwanto, B.H. (2018). Physiological characters and tomato yield under drought stress. IOP Conference Series: Earth and Environmental Science 200 (2018) 012043. https://doi.org/10.1088/1755-1315/200/1/012043.
Silva, K.S., Urban, L.J. K., Balbinota, A., Gnocato, F.S., KRUSE, N.D., Marchesan, E., and Machado, S.L.O. (2016). Electrolyte Leakage and the Protective Effect of Nitric Oxide On Leaves Of Flooded Rice Exposed To Herbicides. Planta Daninha 29(4):837-847. https://doi.org/10.1590/S0100-83582016340400018.
Singh, S.K, Kumar, J. P., Kaur G.S., Datta, S., Singh, V., Dhaka, D., Kapoor, A.B., Wani, D. S., Dhanjal, M., Kumar, L., Harikumar, and J. Singh. (2020). Herbicide Glyphosate: Toxicity and Microbial Degradation. International Journal of Environmental Research and Public Health, 17(20): 7519. https://doi.org/10.3390/ijerph17207519.
Singh, S.K., Barman, M., Prasad J.P., Bahuguna. R.N. (2022). Phenotyping diverse wheat genotypes under terminal heat stress reveal canopy temperature as critical determinant of grain yield. Plant Physiology Reports 27, 335-344. https://doi.org/10.1007/s40502-022-00647-y
Slama, A., Mallek-Maalej, E., Ben Mohamed, H., Rhim, T., Radhouane, L. (2018). A return to the genetic heritage of durum wheat to cope with drought heightened by climate change. PLoS ONE 13(5): e0196873.. https://doi.org/10.1371/journal.pone.0196873.
Soares, C., Pereira, R., Spormann, S., Fidalgo, F. (2019). Is soil contamination by a glyphosate commercial formulation truly harmless to non-target plants - evaluation of oxidative damage and antioxidant responses in tomato. Environmental pollution. 247, 256-265. https://doi.org/10.1016/j.envpol.2019.01.063.
Thakur, V., Rane, J., Nankar, A.N. (2022). Comparative Analysis of Canopy Cooling in wheat under High Temperature and Drought Stress. Agronomy 12(4), 978. https://doi.org/10.3390/agronomy12040978.
Zhu, M., Li F.H., Shi, Z.S. (2016). Morphological and photosynthetic response of waxy corn inbred line to waterlogging. Photosynthetica 54, 636–640. https://doi.org/10.1007/s11099-016-0203-0.
Zobiole, L.H.S., Kremer, R.J., Oliveira, J.R.S., Constantin, J., Oliveira RS. (2011). Glyphosate affects chlorophyll, nodulation and nutrient accumulation of “second generation” glyphosate-resistant soybean (Glycine max L.). Pesticide Biochemistry and Physiology 99, 53–60. https://doi.org/10.1016/j.pestbp.2010.10.005.
Bajji, M., Lutts, S and Kinet, J.M. (2001). Water deficit effects on solute contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions. Plant Science 160(4): 669–681. https://doi.org/10.1016/S0168-9452(00)00443-X.
Bali, A.S., Sidhu, G.P.S. (2019). Abiotic Stress-Induced Oxidative Stress in Wheat. M. Hasanuzzaman et al. (eds.), Wheat Production in Changing Environments, Springer Nature Singapore Pte Ltd. https://doi.org/10.1079/9781789248098.0019.
Caglar, O., Ozturk, A., Aydin, M., Bayram, S. (2011). Paraquat tolerance of bread wheat (Triticum aestivum L.) Genotypes. Journal of animal and veterinary advances 10: 3363-3367. https://doi.org/10.3923/javaa.2011.3363.3367.
Enneb, H., Ben Yahya, L., Ilyas, M., Asaram Dhale, D., Bagues, M and Nagaz, K. (2020). Influence of Water Stress on Growth, Chlorophyll Contents and Solute Accumulation in Three Accessions of Vicia faba L.from Tunisian Arid Region. Abiotic stress in plants. http://dx.doi.org/10.5772/intechopen.94563.
FAO. Food and Agriculture Organization. Crop Prospects and Food Situation - Quarterly Global Report n°. 4, December (2020). Rome. http://www.fao.org/faostat/en/#data/QC.
Freitas-Silva, L., Rodríguez-Ruiz, M., Houmani, H., da Silva, L.C., Palma, J.M., and Corpas F.J. (2017). Glyphosate-induced oxidative stress in Arabidopsis thaliana affecting peroxisomal metabolism and triggers activity in the oxidative phase of the pentose phosphate pathway (OxPPP) involved in NADPH generation. Journal Plant Physiology. 218: 196–205. https://doi.org/10.1016/j.jplph.2017.08.007.
Gomes, M. P., Le Manac’h, S. G., Hénault-Ethier, L., Labrecque, M., Lucotte, M and Juneau, P. (2017). Glyphosate-Dependent Inhibition of Photosynthesis in Willow. Frontiers in Plant Science. 8: 207. https://doi.org/10.3389/fpls.2017.00207.
Gomes, M.P., Smedbol, E., Chalifour, A, Hénault-Ethier, L., Labrecque, M., Lepage, L., Lucotte, M., Juneau, P. (2014). Alteration of plant physiology by glyphosate and its by-product aminomethylphosphonic acid: an overview. Journal of Experimental Botany, Vol. 65, No. 17, pp. 4691–4703. https://doi.org/10.1093/jxb/eru269.
Ibrahim, R.I.H., Alkhudairi, U.A., Alhusayni, S.A.S. (2022). Alleviation of Herbicide Toxicity in Solanum lycopersicum L.-An Antioxidant Stimulation Approach. Plants 2022, 11(17), 2261. https://doi.org/10.3390/plants11172261.
Karabulut, F., and Çanakcı, S. (2021). Effects of Glyphosate Herbicide on Photosynthetic Pigments and Antioxidant Enzyme Activities in Corn (Zea mays L.) and Wheat (Triticum aestivum L.) Varieties. Journal of Physical Chemistry and Functional Materials. Volume 4, Issue 2 (2021) 61-66. https://doi.org/10.54565/jphcfum.1004433.
Khalilzadeh, R., Sharifi, R., and Jalilian, J. (2016). Antioxidant status and physiological responses of wheat (Triticum aestivum L.) to cycocel application and bio fertilizers under water limitation condition. Journal of Plant Interactions, 11:1, 130-137. https://doi.org/10.1080/17429145.2016.1221150.
Malalgoda, M., Ohm, J.B, Howatt, K.A., Simsek, S. (2020). Pre-harvest glyphosate application and effects on wheat starch chemistry: Analysis from application to harvest. Journal of Food Biochemistry. https://doi.org/10.1111/jfbc.13330.
Masoumi, A., Kafi, M., Khazaei, H., Davari, K. (2010). Effect of drought stress on water status, electrolyte leakage and enzymatic antioxidants of kochia (kochia scoparia) under saline condition. Pakistan Journal of Botany 42(5): 3517-3524.
Oulmi, A., and Aissaoui, M.R. (2022). Canopy temperature and chlorophyll content as plant traits indicators for durum wheat (Triticum durum Desf. ) superior lines selection under semi-arid conditions. Agricultural Science and Technology, VOL. 14, (2), 114-122. https://doi.org/10.15547/ast.2022.02.027.
Rivas-Garcia, T., Espinosa-Calderón, A., Hernández-Vázquez, B., and Schwentesius-Rindermann, R. (2022). Overview of Environmental and Health Effects Related to Glyphosate Usage. Sustainability, 14(11), 6868. https://doi.org/10.3390/su14116868.
Sakya, A.T., Sulistyaningsih, E., Indradewa, D., and Purwanto, B.H. (2018). Physiological characters and tomato yield under drought stress. IOP Conference Series: Earth and Environmental Science 200 (2018) 012043. https://doi.org/10.1088/1755-1315/200/1/012043.
Silva, K.S., Urban, L.J. K., Balbinota, A., Gnocato, F.S., KRUSE, N.D., Marchesan, E., and Machado, S.L.O. (2016). Electrolyte Leakage and the Protective Effect of Nitric Oxide On Leaves Of Flooded Rice Exposed To Herbicides. Planta Daninha 29(4):837-847. https://doi.org/10.1590/S0100-83582016340400018.
Singh, S.K, Kumar, J. P., Kaur G.S., Datta, S., Singh, V., Dhaka, D., Kapoor, A.B., Wani, D. S., Dhanjal, M., Kumar, L., Harikumar, and J. Singh. (2020). Herbicide Glyphosate: Toxicity and Microbial Degradation. International Journal of Environmental Research and Public Health, 17(20): 7519. https://doi.org/10.3390/ijerph17207519.
Singh, S.K., Barman, M., Prasad J.P., Bahuguna. R.N. (2022). Phenotyping diverse wheat genotypes under terminal heat stress reveal canopy temperature as critical determinant of grain yield. Plant Physiology Reports 27, 335-344. https://doi.org/10.1007/s40502-022-00647-y
Slama, A., Mallek-Maalej, E., Ben Mohamed, H., Rhim, T., Radhouane, L. (2018). A return to the genetic heritage of durum wheat to cope with drought heightened by climate change. PLoS ONE 13(5): e0196873.. https://doi.org/10.1371/journal.pone.0196873.
Soares, C., Pereira, R., Spormann, S., Fidalgo, F. (2019). Is soil contamination by a glyphosate commercial formulation truly harmless to non-target plants - evaluation of oxidative damage and antioxidant responses in tomato. Environmental pollution. 247, 256-265. https://doi.org/10.1016/j.envpol.2019.01.063.
Thakur, V., Rane, J., Nankar, A.N. (2022). Comparative Analysis of Canopy Cooling in wheat under High Temperature and Drought Stress. Agronomy 12(4), 978. https://doi.org/10.3390/agronomy12040978.
Zhu, M., Li F.H., Shi, Z.S. (2016). Morphological and photosynthetic response of waxy corn inbred line to waterlogging. Photosynthetica 54, 636–640. https://doi.org/10.1007/s11099-016-0203-0.
Zobiole, L.H.S., Kremer, R.J., Oliveira, J.R.S., Constantin, J., Oliveira RS. (2011). Glyphosate affects chlorophyll, nodulation and nutrient accumulation of “second generation” glyphosate-resistant soybean (Glycine max L.). Pesticide Biochemistry and Physiology 99, 53–60. https://doi.org/10.1016/j.pestbp.2010.10.005.
Published
2023-02-04
How to Cite
Benkadja, S., Oulmi, A., Frih, B., Bendada, H., Guendouz, A., & Benmahammed, A. (2023). Evaluation of chlorophyll content and membrane stability under oxidative stress induced by glyphosate herbicide as indicators of drought tolerance in some advanced durum wheat (Triticum durum L.) lines: in vitro study. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 40(1), e234007. Retrieved from https://mail.produccioncientificaluz.org/index.php/agronomia/article/view/39652
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Section
Crop Production
Copyright (c) 2023 Sarah Benkadja, Abdelmalek Oulmi, Benalia Frih, Hocine Bendada, Ali Guendouz4 Amar Benmahammed
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
