Micropropagation of Pelargonium odoratissimum (L.) L’Her. through petioles and leaves

Asghar  Ebrahimzadeh; Maliheh  Fathollahzadeh; Mohammad  Bagher Hassanpouraghdam; Mohammad Ali  Aazami Mavaloo

Asghar Ebrahimzadeh Assitant Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Iran https://orcid.org/0000-0002-8865-6447
Maliheh Fathollahzadeh Former Master student, Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Iran
Mohammad Bagher Hassanpouraghdam Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Iran https://orcid.org/0000-0001-7130-2426
Mohammad Ali Aazami Mavaloo Assitant Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Iran

Keywords: nodal explants, MS medium, shoot organogenesis, BAP, NAA, leaf segments

Abstract

Pelargonium odoratissimum (L.) L’Her is a hard rooting plant and the common methods of propagation via stem cuttings are not successful with this species. therefore, tissue culture methods have been experienced for the mass-propagation of this high-valued species. Intact leaves, leaf segments and petiole sections derived from nodal explants in vitro were employed for the optimization of P. odoratissimum micropropagation. The treatment combinations used were MS and ¹/₂ MS media supplemented with 6-benzylaminopurine, BAP (1, 1.5, 2 and 4.5 mg.L^-1) and 1-naphthaleneacetic acid, NAA (0.1, 1 and 1.5 mg.L^-1). With leaf segments, the lowest browning incidence, the greatest callogenesis and the highest number of shoots were obtained with the media containing 1.5 mg.L^-1 BAP and 1 mg.L^-1 NAA. Two mg.L^-1 BAP + 0.1 mg.L^-1 NAA kept the same results for petiole explants. Intact leaves showed the best results for the three mentioned treatments with 1 mg.L^-1 BAP + 1 mg.L^-1 NAA. 0.2 mg.L^-1 NAA caused the highest rooting percentage and the greatest mean data for the number and length of the roots. Rooted plantlets were transferred to the pots containing 1:1 peat-moss and perlite. Acclimatization of the plantlets was followed by 90 % of survival rate in the greenhouse. The protocol employed would be a potent one to present for the extension section.

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