Micropropagação de Pelargonium odoratissimum (L.) L’Her. através de pecíolos e folhas

• Asghar Ebrahimzadeh Assitant Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Iran https://orcid.org/0000-0002-8865-6447
• Maliheh Fathollahzadeh Former Master student, Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Iran
• Mohammad Bagher Hassanpouraghdam Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Iran https://orcid.org/0000-0001-7130-2426
• Mohammad Ali Aazami Mavaloo Assitant Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, Iran

Palavras-chave: explantes nodais, meio MS, BAP, ANA, organogênese caulinar, segmentos foliares

Resumo

Pelargonium odoratissimum (L.) L’Her é uma planta de enraizamento difícil e os métodos comuns de propagação por meio de estacas não têm sucesso com esta espécie. Portanto, métodos de cultura de tecidos têm sido experimentados para a propagação em massa desta espécie de alto valor. Folhas intactas, segmentos foliares e seções de pecíolo derivados de explantes nodais in vitro foram empregados para a otimização da micropropagação de P. odoratissimum. As combinações de tratamento utilizadas foram meio MS e ¹/₂ MS suplementado com 6-benzilaminopurina, BAP (1, 1,5, 2 e 4,5 mg.L^-1) e ácido 1-naftalenoacético, NAA (0,1, 1 e 1,5 mg.L^{- 1}). Nos segmentos foliares, a menor incidência de escurecimento, a maior calogênese e o maior número de brotações foram obtidos com os meios contendo 1,5 mg.L^-1 de BAP e 1 mg.L^-1 de ANA. Dois mg.L^-1 de BAP + 0,1 mg.L^-1 de NAA mantiveram os mesmos resultados para explantes de pecíolo. As folhas intactas apresentaram os melhores resultados para os três tratamentos citados com 1 mg.L^-1 BAP + 1 mg.L^-1 ANA. 0,2 mg.L^-1 NAA causou a maior porcentagem de enraizamento e os maiores dados médios para o número e comprimento das raízes. As plântulas enraizadas foram transferidas para vasos contendo turfa-musgo 1: 1 e perlita. A aclimatação das mudas foi acompanhada por 90% de sobrevivência em casa de vegetação. O protocolo empregado seria potente para apresentar para a seção de extensão.

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