Isolation and characterization of halotolerant bacteria from soils in the municipality of Remedios, Cuba
Keywords:
bacterial taxonomy, functional characterization, tolerance a salinity
Abstract
The isolation of halotolerant bacteria represents a strategy to mitigate soil salinization and promote crop adaptation. This study aimed to identify bacteria from saline soils in Remedios, Cuba, and evaluate their potential as plant growth-promoting bacteria (PGPB). Three bacterial strains tolerant to 200 mM NaCl were selected and identified as Proteus sp. RM 4.2, Lysinibacillus sp. RM 6.2, and Tenebrionicola sp. RM 4.1. The three strains showed the ability to fix nitrogen and produce siderophores and lytic enzymes. In tomato plant inoculation assays, Proteus sp. RM 4.2 significantly promoted plant growth in the evaluated cultivars, standing out as the strain with the greatest growth-promoting effect. Overall, the results indicated that the evaluated strains exhibited functional traits associated with plant growth promotion and represented a viable alternative for developing bioinoculants in saline soils.
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References
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Alzate-Zuluaga, M.Y., Lima-Milani, K.M., Azeredo- Gonçalves, L.S., & Martinez-de Oliveira, A.L. (2020). Diversity and plant growth-promoting functions of diazotrophic/N-scavenging bacteria isolated from the soils and rhizospheres of two species of Solanum. PLoS ONE, 15(1), e0227422. https://doi.org/10.1371/journal.pone.0227422
Amaresan, N., Jayakumar, V., Kumar, K., & Thajuddin, N. (2021). Plant growth-promoting effects of Proteus mirabilis isolated from tomato (Lycopersicon esculentum Mill) plants. National Academy Science Letters, 44, 453-455. https://doi.org/10.1007/s40009-020-01038-3
Baldani, J.I., Massena-Reis, V., Sampaio-Videira, S., Boddey, L.H., & Divan-Baldani, V.L. (2014). The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiolgists. Plant and Soil, 384, 413-431. https://doi.org/10.1007/s11104-014-2186-6
Campanella, J.J., Bitincka, L., & Smalley, J. (2003). MatGAT: An application that generates similarity/identity matrices using protein or DNA sequences. BMC Bioinformatics, 4, 29. https://doi.org/10.1186/1471-2105-4-29
Darriba, D., Taboada, G. L., Doallo, R., & Posada, D. (2012). jModelTest 2: More models, new heuristics and high-performance computing. Nature Methods, 9(8), 772. https://doi.org/10.1038/nmeth.2109
Dong, L.S., Kim, S.M., Byeon, Y.S., Yang, H.L., Chang S.H., & Kim, I.S. (2022). Tenebrionicola larvae gen. nov., sp. nov., isolated from larvae of mealworm Tenebrio molitor L., and a proposal to transfer Erwinia teleogrylli Liu et al. 2016 to a new genus Entomohabitans as Entomohabitans teleogrylli comb. nov. International Journal of Systematic and Evolutionary Microbiology, 72(4), 005329. https://doi.org/10.1099/ijsem.0.005329
Elbeltagy, A., Nishioka, K., Sato, T., Suzuki, H., Ye, B., Hamada, T., Isawa, T., Mitsui, H., & Minamisawa, K. (2001). Endophytic colonization and in plant a nitrogen fixation by a Herbaspirillum sp. isolated from wild rice species. Applied and Environmental Microbiology, 67(11), 5285-5293. https://doi.org/10.1128/AEM.67.11.5285-5293.2001
García-Reyes, R.A., González-Posada-Dacosta, M.D., Torres-Calzado, K., Villazón-Gómez, J.A., Abellón-MolinaI, M.I., & Velázquez-Sánchez, E.C. (2021). Remote sensing of salinity in agroecosystem of Mayarí, at Holguín Province, Cuba. Revista Ciencias Técnicas Agropecuarias, 30(1), 26-32. https://revistas.unah.edu.cu/index.php/rcta/article/view/1370
Gordon, S.A., & Weber, R.P. (1951). Colorimetric estimation of indoleacetic acid. Plant Physiology, 26(1), 192-195. https://doi.org/10.1104/pp.26.1.192
Guerra-Camacho, M.Á., Magaña-Tzuc, M.C., Vargas-Díaz, A.A., Silva-Rojas, H.V., & Gamboa-Angulo, M. (2024). Identificación y actividad antifúngica de bacterias halófilas aisladas de suelos salinos en Campeche, México. Revista Argentina de Microbiología, 56(3), 298-311. https://doi.org/10.1016/j.ram.2024.02.004
Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
Hernández-Jiménez, A., López-Pérez, D., & Morales-Díaz, M. (2023). Métodos de análisis para calcular el contenido en sales de los suelos de Cuba. Agrotecnia de Cuba, 47(1), 93-97. https://www.agrotecnia.edicionescervantes.com/index.php/agrotecnia/article/view/19
Islam, F., Yasmeen, T., Arif, M.S., Riaz, M., Shahzad, S.M., Imran, Q., & Ali, I. (2016). Combined ability of chromium (Cr) tolerant plant growth-promoting bacteria (PGPB) and salicylic acid (SA) in attenuation of chromium stress in maize plants. Plant Physiology and Biochemistry, 108, 456-467. https://doi.org/10.1016/j.plaphy.2016.08.014
Jalal, A., da Silva-Oliveira, C.E, Shintate-Galindo, F., Leonel-Rosa, P.A., Bueno-Gato, I.M., Horschut-de Lima, B., & Teixera-Filho, M.C. M. (2023). Regulatory mechanisms of plant growth-promoting rhizobacteria and plant nutrition against abiotic stresses in Brassicaceae family. Life, 13(1), 211. https://doi.org/10.3390/life13010211
Jibrin, A.M., Oyewole, O.A., Yakubu, J.G., Hussaini, A., & Egwim, E.C. (2020). Heavy metals biosorption by urease producing Lysinibacillus fusiformis 5B. European Journal of Biological Research, 10(4), 326-335. http://doi.org/10.5281/zenodo.4023161
Jyolsna, K.S., Bharathi, N., Riyaz, L.A., & Paari, P.A. (2021). Impact of Lysinibacillus macroides, a potential plant growth promoting rhizobacteria on growth, yield and nutritional value of tomato plant (Solanum lycopersicum L. f1 hybrid Sachriya). Plant Science Today, 8(2), 365-372. https://doi.org/10.14719/pst.2021.8.2.1082
Kumar, A., Singh, S., Mukherjee, A., Rastogi, R.P., & Verma, J.P. (2021). Salt-tolerant plant growth-promoting Bacillus pumilus strain JPVS11 to enhance plant growth attributes of rice and improve soil health under salinity stress. Microbiological Research, 242, 126616. https://doi.org/10.1016/j.micres.2020.126616
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874. https://doi.org/10.1093/molbev/msw054
Liu, H., Wang, J., Liu, J., Liu, T., & Xue, S. (2021). Hydrogen sulfide (H2S) signaling in plant development and stress responses. aBIOTECH, 2(1), 32-63. https://doi.org/10.1007/s42994-021-00035-4
Louedn, B.C., Haamann, D., & Lynne, A.M. (2011). Use of blue agar CAS assay for siderophore detection. Journal of Microbiology & Biology Education, 12(1), 51-53. https://doi.org/10.1128/jmbe.v12i1.249
Madigan, M.T., Martiniko, J.M., & Parker, J. (2019). Brock Biology of Microorganisms. 15th Ed. Ed. Pearson.
Masood, S., Zhao X.Q., & Shen, R.F. (2020). Bacillus pumilus promotes the growth and nitrogen uptake of tomato plants under nitrogen fertilization. Scientia Horticulturae, 272, 109581. https://doi.org/10.1016/j.scienta.2020.109581
Mathivanan, K., Rajaram, R., & Balasubramanian, V. (2016). Biosorption of Cd (II) and Cu (II) ions using Lysinibacillus fusiformis KMNTT-10: Equilibrium and kinetic studies. Desalination and Water Treatment, 57(47), 22429-22440. https://doi.org/10.1080/19443994.2015.1129508
McFarland, J. (1907). The nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines Journal of the American Medical Association, 49(14), 1176-1178. https://doi.org/10.1001/jama.1907.25320140022001f
Mekibib, B., Abrham, E., Kassaye, A., Tesfaye, D., & Amenu, K. (2015). Clinicopathologic findings associated with urinary tract problems in working donkeys, Southern Ethiopia: A case study. Comparative Clinical Pathology, 24, 417-422. https://doi.org/10.1007/s00580-014-1918-6
Nair, A., Juwarkar, A.A., & Sing, S.K. (2007). Production and characterization of siderophores and its application in arsenic removal from contamined soil. Water, Air, and Soil Pollution, 180, 199-212. https://doi.org/10.1007/s11270-006-9263-2
Nautiyal, S.C. (1999). An efficient microbiological growth medium for screening phosphate solubilizing microorganism. FEMS Microbiology Letters, 170(1), 265-270. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
Passera, A., Rossato, M., Oliver, J.S., Battelli, G., Shahzad, G.I.R., Cosentino, E., Sage, J.M., Toffolatti, S.L., Lopatriello, G., Davis, J.R., Kaiser, M.D., Delledonne, M., & Casati, P. (2021). Characterization of Lysinibacillus fusiformis strain S4C11: In vitro, in planta, and in silico analyses reveal a plant-beneficial microbe. Microbiological Research, 244, 126665. https://doi.org/10.1016/j.micres.2020.126665
Pineda-Ruiz, E., González-Hidalgo, M., de León-Ortiz, M.E., Villegas-Delgado, R., Más-Martínez, R., & Mora-Varona, R. (2023). Variación del carbono orgánico en suelo Pardo mullido carbonatado monocultivado con caña de azúcar durante 35 años en Cuba. Ingeniería Agrícola, 13(1), 35-40. https://revistas.unah.edu.cu/index.php/IAgric/article/view/1668
Ramos-Garza, J., Bustamante-Brito, R., Angeles-de Paz, G., Medina-Canales, M.G., Vásquez-Murrieta, M.S., Tao-Wang, E., & Rodríguez-Tovar, V.A. (2016). Isolation and characterization of yeasts associated with plants growing in heavy-metal and arsenic-contaminated soils, Canadian Journal of Microbiology, 62(4), 307-319. https://doi.org/10.1139/cjm-2015-0226
Rodríguez-Martinez, C.F., & Zhurbenko, R. (2018). Manual Biocen de medios de cultivo. (4.a ed.). Centro Nacional de Biopreparados (BIOCEN). https://www.biocen.cu/wp-content/uploads/2021/05/Manual-MC-2018.pdf
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Published
2026-03-05
How to Cite
Ortega-Garcia, M., Ríos-Rocafull, Y., Tejeda-González, G., Zelaya-Molina, L., Chávez-Díaz, I., & Nápoles-García, M. (2026). Isolation and characterization of halotolerant bacteria from soils in the municipality of Remedios, Cuba. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 43(1), e264318. Retrieved from http://mail.produccioncientificaluz.org/index.php/agronomia/article/view/45269
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Section
Crop Production
Copyright (c) 2026 Marisel Ortega-García, Yoania Ríos-Rocafull, Grisel Tejeda-González, Lily Zelaya-Molina, Ismael Chávez-Díaz, María Nápoles-García
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
