Isolamento e caracterização de bactérias halotolerantes provenientes de solos do município Remedios, Cuba
Palavras-chave:
taxonomia bacteriana, caracterização funcional, tolerância à salinidade
Resumo
O isolamento de bactérias halotolerantes representa uma estratégia para mitigar a salinização do solo e favorecer a adaptação das culturas. Este estudo teve como objetivo identificar bactérias de solos salinos en Remedios, Cuba, e avaliar seu potencial como promotoras de crescimento vegetal (PGPB). Três cepas bacterianas tolerantes a 200 mM de NaCl foram selecionadas e identificadas como Proteus sp. RM 4.2, Lysinibacillus sp. RM 6.2 e Tenebrionicola sp. RM 4.1. As três cepas apresentaram capacidade de fixar nitrogênio e de produzir sideróforos e enzimas líticas. Em ensaios de inoculação em plantas de tomate, Proteus sp. RM 4.2 promoveu significativamente o crescimento vegetal nos cultivares avaliados, destacando-se como a cepa com maior efeito promotor. Em conjunto, os resultados indicam que as cepas avaliadas apresentam características funcionais associadas à promoção do crescimento vegetal e representam uma alternativa viável para o desenvolvimento de bioinoculantes em solos salinos.
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Referências
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Al-Mohaini, M., Farid, A., Muzammal, M., Ghazanfar, S., Dadrasnia, A., Alsalman, A.J., Al-Hawaj, M.A., Alhashem, Y.N., & Ismail, S. (2022). Enhancing lipase production of Bacillus salmalaya strain 139SI using different carbon sources and surfactants. Applied Microbiology, 2(1), 237-247. https://doi.org/10.3390/applmicrobiol2010017
Alzate-Zuluaga, M.Y., Lima-Milani, K.M., Azeredo- Gonçalves, L.S., & Martinez-de Oliveira, A.L. (2020). Diversity and plant growth-promoting functions of diazotrophic/N-scavenging bacteria isolated from the soils and rhizospheres of two species of Solanum. PLoS ONE, 15(1), e0227422. https://doi.org/10.1371/journal.pone.0227422
Amaresan, N., Jayakumar, V., Kumar, K., & Thajuddin, N. (2021). Plant growth-promoting effects of Proteus mirabilis isolated from tomato (Lycopersicon esculentum Mill) plants. National Academy Science Letters, 44, 453-455. https://doi.org/10.1007/s40009-020-01038-3
Baldani, J.I., Massena-Reis, V., Sampaio-Videira, S., Boddey, L.H., & Divan-Baldani, V.L. (2014). The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiolgists. Plant and Soil, 384, 413-431. https://doi.org/10.1007/s11104-014-2186-6
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Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
Hernández-Jiménez, A., López-Pérez, D., & Morales-Díaz, M. (2023). Métodos de análisis para calcular el contenido en sales de los suelos de Cuba. Agrotecnia de Cuba, 47(1), 93-97. https://www.agrotecnia.edicionescervantes.com/index.php/agrotecnia/article/view/19
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Jalal, A., da Silva-Oliveira, C.E, Shintate-Galindo, F., Leonel-Rosa, P.A., Bueno-Gato, I.M., Horschut-de Lima, B., & Teixera-Filho, M.C. M. (2023). Regulatory mechanisms of plant growth-promoting rhizobacteria and plant nutrition against abiotic stresses in Brassicaceae family. Life, 13(1), 211. https://doi.org/10.3390/life13010211
Jibrin, A.M., Oyewole, O.A., Yakubu, J.G., Hussaini, A., & Egwim, E.C. (2020). Heavy metals biosorption by urease producing Lysinibacillus fusiformis 5B. European Journal of Biological Research, 10(4), 326-335. http://doi.org/10.5281/zenodo.4023161
Jyolsna, K.S., Bharathi, N., Riyaz, L.A., & Paari, P.A. (2021). Impact of Lysinibacillus macroides, a potential plant growth promoting rhizobacteria on growth, yield and nutritional value of tomato plant (Solanum lycopersicum L. f1 hybrid Sachriya). Plant Science Today, 8(2), 365-372. https://doi.org/10.14719/pst.2021.8.2.1082
Kumar, A., Singh, S., Mukherjee, A., Rastogi, R.P., & Verma, J.P. (2021). Salt-tolerant plant growth-promoting Bacillus pumilus strain JPVS11 to enhance plant growth attributes of rice and improve soil health under salinity stress. Microbiological Research, 242, 126616. https://doi.org/10.1016/j.micres.2020.126616
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874. https://doi.org/10.1093/molbev/msw054
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Mathivanan, K., Rajaram, R., & Balasubramanian, V. (2016). Biosorption of Cd (II) and Cu (II) ions using Lysinibacillus fusiformis KMNTT-10: Equilibrium and kinetic studies. Desalination and Water Treatment, 57(47), 22429-22440. https://doi.org/10.1080/19443994.2015.1129508
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Mekibib, B., Abrham, E., Kassaye, A., Tesfaye, D., & Amenu, K. (2015). Clinicopathologic findings associated with urinary tract problems in working donkeys, Southern Ethiopia: A case study. Comparative Clinical Pathology, 24, 417-422. https://doi.org/10.1007/s00580-014-1918-6
Nair, A., Juwarkar, A.A., & Sing, S.K. (2007). Production and characterization of siderophores and its application in arsenic removal from contamined soil. Water, Air, and Soil Pollution, 180, 199-212. https://doi.org/10.1007/s11270-006-9263-2
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Akintokun, A.K., Ezaka, E., Akintokun, P.O., Shittu, O.B., & Taiwo, L.B. (2019). Isolation, screening and response of maize to plant growth promoting Rhizobacteria inoculants. Scientia Agriculturae Bohemica, 50(3), 181-190. https://sab.czu.cz/en/r-8044-archive/r-14574-2019-issues/r-14577-03-2019/isolation-screening-and-response-of-maize-to-plant-growth-pr.html
Al-Mohaini, M., Farid, A., Muzammal, M., Ghazanfar, S., Dadrasnia, A., Alsalman, A.J., Al-Hawaj, M.A., Alhashem, Y.N., & Ismail, S. (2022). Enhancing lipase production of Bacillus salmalaya strain 139SI using different carbon sources and surfactants. Applied Microbiology, 2(1), 237-247. https://doi.org/10.3390/applmicrobiol2010017
Alzate-Zuluaga, M.Y., Lima-Milani, K.M., Azeredo- Gonçalves, L.S., & Martinez-de Oliveira, A.L. (2020). Diversity and plant growth-promoting functions of diazotrophic/N-scavenging bacteria isolated from the soils and rhizospheres of two species of Solanum. PLoS ONE, 15(1), e0227422. https://doi.org/10.1371/journal.pone.0227422
Amaresan, N., Jayakumar, V., Kumar, K., & Thajuddin, N. (2021). Plant growth-promoting effects of Proteus mirabilis isolated from tomato (Lycopersicon esculentum Mill) plants. National Academy Science Letters, 44, 453-455. https://doi.org/10.1007/s40009-020-01038-3
Baldani, J.I., Massena-Reis, V., Sampaio-Videira, S., Boddey, L.H., & Divan-Baldani, V.L. (2014). The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiolgists. Plant and Soil, 384, 413-431. https://doi.org/10.1007/s11104-014-2186-6
Campanella, J.J., Bitincka, L., & Smalley, J. (2003). MatGAT: An application that generates similarity/identity matrices using protein or DNA sequences. BMC Bioinformatics, 4, 29. https://doi.org/10.1186/1471-2105-4-29
Darriba, D., Taboada, G. L., Doallo, R., & Posada, D. (2012). jModelTest 2: More models, new heuristics and high-performance computing. Nature Methods, 9(8), 772. https://doi.org/10.1038/nmeth.2109
Dong, L.S., Kim, S.M., Byeon, Y.S., Yang, H.L., Chang S.H., & Kim, I.S. (2022). Tenebrionicola larvae gen. nov., sp. nov., isolated from larvae of mealworm Tenebrio molitor L., and a proposal to transfer Erwinia teleogrylli Liu et al. 2016 to a new genus Entomohabitans as Entomohabitans teleogrylli comb. nov. International Journal of Systematic and Evolutionary Microbiology, 72(4), 005329. https://doi.org/10.1099/ijsem.0.005329
Elbeltagy, A., Nishioka, K., Sato, T., Suzuki, H., Ye, B., Hamada, T., Isawa, T., Mitsui, H., & Minamisawa, K. (2001). Endophytic colonization and in plant a nitrogen fixation by a Herbaspirillum sp. isolated from wild rice species. Applied and Environmental Microbiology, 67(11), 5285-5293. https://doi.org/10.1128/AEM.67.11.5285-5293.2001
García-Reyes, R.A., González-Posada-Dacosta, M.D., Torres-Calzado, K., Villazón-Gómez, J.A., Abellón-MolinaI, M.I., & Velázquez-Sánchez, E.C. (2021). Remote sensing of salinity in agroecosystem of Mayarí, at Holguín Province, Cuba. Revista Ciencias Técnicas Agropecuarias, 30(1), 26-32. https://revistas.unah.edu.cu/index.php/rcta/article/view/1370
Gordon, S.A., & Weber, R.P. (1951). Colorimetric estimation of indoleacetic acid. Plant Physiology, 26(1), 192-195. https://doi.org/10.1104/pp.26.1.192
Guerra-Camacho, M.Á., Magaña-Tzuc, M.C., Vargas-Díaz, A.A., Silva-Rojas, H.V., & Gamboa-Angulo, M. (2024). Identificación y actividad antifúngica de bacterias halófilas aisladas de suelos salinos en Campeche, México. Revista Argentina de Microbiología, 56(3), 298-311. https://doi.org/10.1016/j.ram.2024.02.004
Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
Hernández-Jiménez, A., López-Pérez, D., & Morales-Díaz, M. (2023). Métodos de análisis para calcular el contenido en sales de los suelos de Cuba. Agrotecnia de Cuba, 47(1), 93-97. https://www.agrotecnia.edicionescervantes.com/index.php/agrotecnia/article/view/19
Islam, F., Yasmeen, T., Arif, M.S., Riaz, M., Shahzad, S.M., Imran, Q., & Ali, I. (2016). Combined ability of chromium (Cr) tolerant plant growth-promoting bacteria (PGPB) and salicylic acid (SA) in attenuation of chromium stress in maize plants. Plant Physiology and Biochemistry, 108, 456-467. https://doi.org/10.1016/j.plaphy.2016.08.014
Jalal, A., da Silva-Oliveira, C.E, Shintate-Galindo, F., Leonel-Rosa, P.A., Bueno-Gato, I.M., Horschut-de Lima, B., & Teixera-Filho, M.C. M. (2023). Regulatory mechanisms of plant growth-promoting rhizobacteria and plant nutrition against abiotic stresses in Brassicaceae family. Life, 13(1), 211. https://doi.org/10.3390/life13010211
Jibrin, A.M., Oyewole, O.A., Yakubu, J.G., Hussaini, A., & Egwim, E.C. (2020). Heavy metals biosorption by urease producing Lysinibacillus fusiformis 5B. European Journal of Biological Research, 10(4), 326-335. http://doi.org/10.5281/zenodo.4023161
Jyolsna, K.S., Bharathi, N., Riyaz, L.A., & Paari, P.A. (2021). Impact of Lysinibacillus macroides, a potential plant growth promoting rhizobacteria on growth, yield and nutritional value of tomato plant (Solanum lycopersicum L. f1 hybrid Sachriya). Plant Science Today, 8(2), 365-372. https://doi.org/10.14719/pst.2021.8.2.1082
Kumar, A., Singh, S., Mukherjee, A., Rastogi, R.P., & Verma, J.P. (2021). Salt-tolerant plant growth-promoting Bacillus pumilus strain JPVS11 to enhance plant growth attributes of rice and improve soil health under salinity stress. Microbiological Research, 242, 126616. https://doi.org/10.1016/j.micres.2020.126616
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874. https://doi.org/10.1093/molbev/msw054
Liu, H., Wang, J., Liu, J., Liu, T., & Xue, S. (2021). Hydrogen sulfide (H2S) signaling in plant development and stress responses. aBIOTECH, 2(1), 32-63. https://doi.org/10.1007/s42994-021-00035-4
Louedn, B.C., Haamann, D., & Lynne, A.M. (2011). Use of blue agar CAS assay for siderophore detection. Journal of Microbiology & Biology Education, 12(1), 51-53. https://doi.org/10.1128/jmbe.v12i1.249
Madigan, M.T., Martiniko, J.M., & Parker, J. (2019). Brock Biology of Microorganisms. 15th Ed. Ed. Pearson.
Masood, S., Zhao X.Q., & Shen, R.F. (2020). Bacillus pumilus promotes the growth and nitrogen uptake of tomato plants under nitrogen fertilization. Scientia Horticulturae, 272, 109581. https://doi.org/10.1016/j.scienta.2020.109581
Mathivanan, K., Rajaram, R., & Balasubramanian, V. (2016). Biosorption of Cd (II) and Cu (II) ions using Lysinibacillus fusiformis KMNTT-10: Equilibrium and kinetic studies. Desalination and Water Treatment, 57(47), 22429-22440. https://doi.org/10.1080/19443994.2015.1129508
McFarland, J. (1907). The nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines Journal of the American Medical Association, 49(14), 1176-1178. https://doi.org/10.1001/jama.1907.25320140022001f
Mekibib, B., Abrham, E., Kassaye, A., Tesfaye, D., & Amenu, K. (2015). Clinicopathologic findings associated with urinary tract problems in working donkeys, Southern Ethiopia: A case study. Comparative Clinical Pathology, 24, 417-422. https://doi.org/10.1007/s00580-014-1918-6
Nair, A., Juwarkar, A.A., & Sing, S.K. (2007). Production and characterization of siderophores and its application in arsenic removal from contamined soil. Water, Air, and Soil Pollution, 180, 199-212. https://doi.org/10.1007/s11270-006-9263-2
Nautiyal, S.C. (1999). An efficient microbiological growth medium for screening phosphate solubilizing microorganism. FEMS Microbiology Letters, 170(1), 265-270. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
Passera, A., Rossato, M., Oliver, J.S., Battelli, G., Shahzad, G.I.R., Cosentino, E., Sage, J.M., Toffolatti, S.L., Lopatriello, G., Davis, J.R., Kaiser, M.D., Delledonne, M., & Casati, P. (2021). Characterization of Lysinibacillus fusiformis strain S4C11: In vitro, in planta, and in silico analyses reveal a plant-beneficial microbe. Microbiological Research, 244, 126665. https://doi.org/10.1016/j.micres.2020.126665
Pineda-Ruiz, E., González-Hidalgo, M., de León-Ortiz, M.E., Villegas-Delgado, R., Más-Martínez, R., & Mora-Varona, R. (2023). Variación del carbono orgánico en suelo Pardo mullido carbonatado monocultivado con caña de azúcar durante 35 años en Cuba. Ingeniería Agrícola, 13(1), 35-40. https://revistas.unah.edu.cu/index.php/IAgric/article/view/1668
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Publicado
2026-03-05
Como Citar
Ortega-Garcia, M., Ríos-Rocafull, Y., Tejeda-González, G., Zelaya-Molina, L., Chávez-Díaz, I., & Nápoles-García, M. (2026). Isolamento e caracterização de bactérias halotolerantes provenientes de solos do município Remedios, Cuba. Revista Da Faculdade De Agronomia Da Universidade De Zulia, 43(1), e264318. Obtido de http://mail.produccioncientificaluz.org/index.php/agronomia/article/view/45269
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Direitos de Autor (c) 2026 Marisel Ortega-García, Yoania Ríos-Rocafull, Grisel Tejeda-González, Lily Zelaya-Molina, Ismael Chávez-Díaz, María Nápoles-García
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