Aislamiento y caracterización de bacterias halotolerantes procedentes de suelos del municipio Remedios, Cuba
Palabras clave:
taxonomía bacteriana, caracterización funcional, tolerancia a salinidad
Resumen
El aislamiento de bacterias halotolerantes representa una estrategia para mitigar la salinización del suelo y favorecer la adaptación de los cultivos. Este estudio tuvo como objetivo identificar bacterias de suelos salinos en Remedios, Cuba, y evaluar su potencial como promotoras de crecimiento vegetal (PGPB). Se seleccionaron tres cepas bacterianas tolerantes a 200 mM de NaCl, identificadas como Proteus sp. RM 4.2, Lysinibacillus sp. RM 6.2 y Tenebrionicola sp. RM 4.1. Las tres cepas presentaron capacidad de fijar nitrógeno, producir sideróforos y enzimas líticas. En ensayos de inoculación en plantas de tomate, Proteus sp. RM 4.2 promovió significativamente el crecimiento vegetal en los cultivares evaluados, destacándose como la cepa con mayor efecto promotor de crecimiento. En conjunto, los resultados indicaron que las cepas evaluadas presentaron rasgos funcionales asociados a la promoción del crecimiento vegetal y representan una alternativa viable para el desarrollo de bioinoculantes en suelos salinos.
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Alzate-Zuluaga, M.Y., Lima-Milani, K.M., Azeredo- Gonçalves, L.S., & Martinez-de Oliveira, A.L. (2020). Diversity and plant growth-promoting functions of diazotrophic/N-scavenging bacteria isolated from the soils and rhizospheres of two species of Solanum. PLoS ONE, 15(1), e0227422. https://doi.org/10.1371/journal.pone.0227422
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Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
Hernández-Jiménez, A., López-Pérez, D., & Morales-Díaz, M. (2023). Métodos de análisis para calcular el contenido en sales de los suelos de Cuba. Agrotecnia de Cuba, 47(1), 93-97. https://www.agrotecnia.edicionescervantes.com/index.php/agrotecnia/article/view/19
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Jalal, A., da Silva-Oliveira, C.E, Shintate-Galindo, F., Leonel-Rosa, P.A., Bueno-Gato, I.M., Horschut-de Lima, B., & Teixera-Filho, M.C. M. (2023). Regulatory mechanisms of plant growth-promoting rhizobacteria and plant nutrition against abiotic stresses in Brassicaceae family. Life, 13(1), 211. https://doi.org/10.3390/life13010211
Jibrin, A.M., Oyewole, O.A., Yakubu, J.G., Hussaini, A., & Egwim, E.C. (2020). Heavy metals biosorption by urease producing Lysinibacillus fusiformis 5B. European Journal of Biological Research, 10(4), 326-335. http://doi.org/10.5281/zenodo.4023161
Jyolsna, K.S., Bharathi, N., Riyaz, L.A., & Paari, P.A. (2021). Impact of Lysinibacillus macroides, a potential plant growth promoting rhizobacteria on growth, yield and nutritional value of tomato plant (Solanum lycopersicum L. f1 hybrid Sachriya). Plant Science Today, 8(2), 365-372. https://doi.org/10.14719/pst.2021.8.2.1082
Kumar, A., Singh, S., Mukherjee, A., Rastogi, R.P., & Verma, J.P. (2021). Salt-tolerant plant growth-promoting Bacillus pumilus strain JPVS11 to enhance plant growth attributes of rice and improve soil health under salinity stress. Microbiological Research, 242, 126616. https://doi.org/10.1016/j.micres.2020.126616
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Liu, H., Wang, J., Liu, J., Liu, T., & Xue, S. (2021). Hydrogen sulfide (H2S) signaling in plant development and stress responses. aBIOTECH, 2(1), 32-63. https://doi.org/10.1007/s42994-021-00035-4
Louedn, B.C., Haamann, D., & Lynne, A.M. (2011). Use of blue agar CAS assay for siderophore detection. Journal of Microbiology & Biology Education, 12(1), 51-53. https://doi.org/10.1128/jmbe.v12i1.249
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Mathivanan, K., Rajaram, R., & Balasubramanian, V. (2016). Biosorption of Cd (II) and Cu (II) ions using Lysinibacillus fusiformis KMNTT-10: Equilibrium and kinetic studies. Desalination and Water Treatment, 57(47), 22429-22440. https://doi.org/10.1080/19443994.2015.1129508
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Mekibib, B., Abrham, E., Kassaye, A., Tesfaye, D., & Amenu, K. (2015). Clinicopathologic findings associated with urinary tract problems in working donkeys, Southern Ethiopia: A case study. Comparative Clinical Pathology, 24, 417-422. https://doi.org/10.1007/s00580-014-1918-6
Nair, A., Juwarkar, A.A., & Sing, S.K. (2007). Production and characterization of siderophores and its application in arsenic removal from contamined soil. Water, Air, and Soil Pollution, 180, 199-212. https://doi.org/10.1007/s11270-006-9263-2
Nautiyal, S.C. (1999). An efficient microbiological growth medium for screening phosphate solubilizing microorganism. FEMS Microbiology Letters, 170(1), 265-270. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
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Akintokun, A.K., Ezaka, E., Akintokun, P.O., Shittu, O.B., & Taiwo, L.B. (2019). Isolation, screening and response of maize to plant growth promoting Rhizobacteria inoculants. Scientia Agriculturae Bohemica, 50(3), 181-190. https://sab.czu.cz/en/r-8044-archive/r-14574-2019-issues/r-14577-03-2019/isolation-screening-and-response-of-maize-to-plant-growth-pr.html
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Alzate-Zuluaga, M.Y., Lima-Milani, K.M., Azeredo- Gonçalves, L.S., & Martinez-de Oliveira, A.L. (2020). Diversity and plant growth-promoting functions of diazotrophic/N-scavenging bacteria isolated from the soils and rhizospheres of two species of Solanum. PLoS ONE, 15(1), e0227422. https://doi.org/10.1371/journal.pone.0227422
Amaresan, N., Jayakumar, V., Kumar, K., & Thajuddin, N. (2021). Plant growth-promoting effects of Proteus mirabilis isolated from tomato (Lycopersicon esculentum Mill) plants. National Academy Science Letters, 44, 453-455. https://doi.org/10.1007/s40009-020-01038-3
Baldani, J.I., Massena-Reis, V., Sampaio-Videira, S., Boddey, L.H., & Divan-Baldani, V.L. (2014). The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiolgists. Plant and Soil, 384, 413-431. https://doi.org/10.1007/s11104-014-2186-6
Campanella, J.J., Bitincka, L., & Smalley, J. (2003). MatGAT: An application that generates similarity/identity matrices using protein or DNA sequences. BMC Bioinformatics, 4, 29. https://doi.org/10.1186/1471-2105-4-29
Darriba, D., Taboada, G. L., Doallo, R., & Posada, D. (2012). jModelTest 2: More models, new heuristics and high-performance computing. Nature Methods, 9(8), 772. https://doi.org/10.1038/nmeth.2109
Dong, L.S., Kim, S.M., Byeon, Y.S., Yang, H.L., Chang S.H., & Kim, I.S. (2022). Tenebrionicola larvae gen. nov., sp. nov., isolated from larvae of mealworm Tenebrio molitor L., and a proposal to transfer Erwinia teleogrylli Liu et al. 2016 to a new genus Entomohabitans as Entomohabitans teleogrylli comb. nov. International Journal of Systematic and Evolutionary Microbiology, 72(4), 005329. https://doi.org/10.1099/ijsem.0.005329
Elbeltagy, A., Nishioka, K., Sato, T., Suzuki, H., Ye, B., Hamada, T., Isawa, T., Mitsui, H., & Minamisawa, K. (2001). Endophytic colonization and in plant a nitrogen fixation by a Herbaspirillum sp. isolated from wild rice species. Applied and Environmental Microbiology, 67(11), 5285-5293. https://doi.org/10.1128/AEM.67.11.5285-5293.2001
García-Reyes, R.A., González-Posada-Dacosta, M.D., Torres-Calzado, K., Villazón-Gómez, J.A., Abellón-MolinaI, M.I., & Velázquez-Sánchez, E.C. (2021). Remote sensing of salinity in agroecosystem of Mayarí, at Holguín Province, Cuba. Revista Ciencias Técnicas Agropecuarias, 30(1), 26-32. https://revistas.unah.edu.cu/index.php/rcta/article/view/1370
Gordon, S.A., & Weber, R.P. (1951). Colorimetric estimation of indoleacetic acid. Plant Physiology, 26(1), 192-195. https://doi.org/10.1104/pp.26.1.192
Guerra-Camacho, M.Á., Magaña-Tzuc, M.C., Vargas-Díaz, A.A., Silva-Rojas, H.V., & Gamboa-Angulo, M. (2024). Identificación y actividad antifúngica de bacterias halófilas aisladas de suelos salinos en Campeche, México. Revista Argentina de Microbiología, 56(3), 298-311. https://doi.org/10.1016/j.ram.2024.02.004
Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
Hernández-Jiménez, A., López-Pérez, D., & Morales-Díaz, M. (2023). Métodos de análisis para calcular el contenido en sales de los suelos de Cuba. Agrotecnia de Cuba, 47(1), 93-97. https://www.agrotecnia.edicionescervantes.com/index.php/agrotecnia/article/view/19
Islam, F., Yasmeen, T., Arif, M.S., Riaz, M., Shahzad, S.M., Imran, Q., & Ali, I. (2016). Combined ability of chromium (Cr) tolerant plant growth-promoting bacteria (PGPB) and salicylic acid (SA) in attenuation of chromium stress in maize plants. Plant Physiology and Biochemistry, 108, 456-467. https://doi.org/10.1016/j.plaphy.2016.08.014
Jalal, A., da Silva-Oliveira, C.E, Shintate-Galindo, F., Leonel-Rosa, P.A., Bueno-Gato, I.M., Horschut-de Lima, B., & Teixera-Filho, M.C. M. (2023). Regulatory mechanisms of plant growth-promoting rhizobacteria and plant nutrition against abiotic stresses in Brassicaceae family. Life, 13(1), 211. https://doi.org/10.3390/life13010211
Jibrin, A.M., Oyewole, O.A., Yakubu, J.G., Hussaini, A., & Egwim, E.C. (2020). Heavy metals biosorption by urease producing Lysinibacillus fusiformis 5B. European Journal of Biological Research, 10(4), 326-335. http://doi.org/10.5281/zenodo.4023161
Jyolsna, K.S., Bharathi, N., Riyaz, L.A., & Paari, P.A. (2021). Impact of Lysinibacillus macroides, a potential plant growth promoting rhizobacteria on growth, yield and nutritional value of tomato plant (Solanum lycopersicum L. f1 hybrid Sachriya). Plant Science Today, 8(2), 365-372. https://doi.org/10.14719/pst.2021.8.2.1082
Kumar, A., Singh, S., Mukherjee, A., Rastogi, R.P., & Verma, J.P. (2021). Salt-tolerant plant growth-promoting Bacillus pumilus strain JPVS11 to enhance plant growth attributes of rice and improve soil health under salinity stress. Microbiological Research, 242, 126616. https://doi.org/10.1016/j.micres.2020.126616
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874. https://doi.org/10.1093/molbev/msw054
Liu, H., Wang, J., Liu, J., Liu, T., & Xue, S. (2021). Hydrogen sulfide (H2S) signaling in plant development and stress responses. aBIOTECH, 2(1), 32-63. https://doi.org/10.1007/s42994-021-00035-4
Louedn, B.C., Haamann, D., & Lynne, A.M. (2011). Use of blue agar CAS assay for siderophore detection. Journal of Microbiology & Biology Education, 12(1), 51-53. https://doi.org/10.1128/jmbe.v12i1.249
Madigan, M.T., Martiniko, J.M., & Parker, J. (2019). Brock Biology of Microorganisms. 15th Ed. Ed. Pearson.
Masood, S., Zhao X.Q., & Shen, R.F. (2020). Bacillus pumilus promotes the growth and nitrogen uptake of tomato plants under nitrogen fertilization. Scientia Horticulturae, 272, 109581. https://doi.org/10.1016/j.scienta.2020.109581
Mathivanan, K., Rajaram, R., & Balasubramanian, V. (2016). Biosorption of Cd (II) and Cu (II) ions using Lysinibacillus fusiformis KMNTT-10: Equilibrium and kinetic studies. Desalination and Water Treatment, 57(47), 22429-22440. https://doi.org/10.1080/19443994.2015.1129508
McFarland, J. (1907). The nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines Journal of the American Medical Association, 49(14), 1176-1178. https://doi.org/10.1001/jama.1907.25320140022001f
Mekibib, B., Abrham, E., Kassaye, A., Tesfaye, D., & Amenu, K. (2015). Clinicopathologic findings associated with urinary tract problems in working donkeys, Southern Ethiopia: A case study. Comparative Clinical Pathology, 24, 417-422. https://doi.org/10.1007/s00580-014-1918-6
Nair, A., Juwarkar, A.A., & Sing, S.K. (2007). Production and characterization of siderophores and its application in arsenic removal from contamined soil. Water, Air, and Soil Pollution, 180, 199-212. https://doi.org/10.1007/s11270-006-9263-2
Nautiyal, S.C. (1999). An efficient microbiological growth medium for screening phosphate solubilizing microorganism. FEMS Microbiology Letters, 170(1), 265-270. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
Passera, A., Rossato, M., Oliver, J.S., Battelli, G., Shahzad, G.I.R., Cosentino, E., Sage, J.M., Toffolatti, S.L., Lopatriello, G., Davis, J.R., Kaiser, M.D., Delledonne, M., & Casati, P. (2021). Characterization of Lysinibacillus fusiformis strain S4C11: In vitro, in planta, and in silico analyses reveal a plant-beneficial microbe. Microbiological Research, 244, 126665. https://doi.org/10.1016/j.micres.2020.126665
Pineda-Ruiz, E., González-Hidalgo, M., de León-Ortiz, M.E., Villegas-Delgado, R., Más-Martínez, R., & Mora-Varona, R. (2023). Variación del carbono orgánico en suelo Pardo mullido carbonatado monocultivado con caña de azúcar durante 35 años en Cuba. Ingeniería Agrícola, 13(1), 35-40. https://revistas.unah.edu.cu/index.php/IAgric/article/view/1668
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Publicado
2026-03-05
Cómo citar
Ortega-Garcia, M., Ríos-Rocafull, Y., Tejeda-González, G., Zelaya-Molina, L., Chávez-Díaz, I., & Nápoles-García, M. (2026). Aislamiento y caracterización de bacterias halotolerantes procedentes de suelos del municipio Remedios, Cuba. Revista De La Facultad De Agronomía De La Universidad Del Zulia, 43(1), e264318. Recuperado a partir de http://mail.produccioncientificaluz.org/index.php/agronomia/article/view/45269
Número
Sección
Producción Vegetal
Derechos de autor 2026 Marisel Ortega-García, Yoania Ríos-Rocafull, Grisel Tejeda-González, Lily Zelaya-Molina, Ismael Chávez-Díaz, María Nápoles-García
Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
